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Effects of phenobarbital upon bile acid synthesis in two strains of rats

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Lipids

Abstract

Rats of the Wistar and Sprague-Dawley strains were injected with sodium phenobarbital (100 mg/kg body wt/day) for 8 days. Fecal bile acid excretion was measured on days 6 and 8 of the experiment, and biliary bile acid composition, hepatic microsomal cholesterol, 7α-hydroxylase, and 7α-hydroxy-4-cholesten-3-one 12α-hydroxylase were determined at the end of the study. In the Wistar rat, injection of phenobarbital produced a doubling of fecal bile acid output (controls, 5.3 mg/rat/day; treated rats, 10.6 mg/rat/day) and a two-three fold increase in cholesterol 7α-hydroxylase. The fecal bile acid output of Sprague-Dawley rats increased 20% in response to phenobarbital (controls, 9.5 mg/rat/day; treated rats, 11.6 mg/rat/day). The activity of cholesterol 7α-hydroxylase remained unchanged. In both strains, phenobarbital treatment produced a decrease in the proportion of cholic acid in total biliary bile acids (controls, 85%; treated groups, 65%). This was associated with a decrease of 7α-hydroxy-4-cholesten-3-one 12α-hydroxylase activity by ca. 50%. Biliary cholesterol concentrations were reduced in phenobarbital treated rats of both strains, but liver cholesterol concentrations remained unchanged. The drug produced a 25% increase in liver wt, on the average.

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References

  1. Conney, A.H., and J.J. Burns, Adv. Pharmacol. 1:31 (1962).

    CAS  Google Scholar 

  2. Kuntzman, R., Ann. Rev. Pharmacol. 9:21 (1969).

    Article  PubMed  CAS  Google Scholar 

  3. Shefer, S., S. Hauser, V. Lapar, and E.H. Mosbach, J. Lipid Res. 14:573 (1973).

    PubMed  CAS  Google Scholar 

  4. Boyd, G.S., and I.W. Percey-Robb, Amer. J. Med. 51:580 (1971).

    Article  PubMed  CAS  Google Scholar 

  5. Einarsson, K., and G. Johansson, Eur. J. Biochem. 6:293 (1968).

    Article  PubMed  CAS  Google Scholar 

  6. Shefer, S., S. Hauser, and E.H. Mosbach, J. Lipid Res. 13:69 (1972).

    PubMed  CAS  Google Scholar 

  7. Wada, F., K. Hirata, K. Nakao, and Y. Sakamoto, J. Biochem. 64:415 (1968).

    PubMed  CAS  Google Scholar 

  8. Mitropoulos, K.A., M. Suzuki, N.B. Myant, and H. Danielsson, FEBS Lett. 1:13 (1968).

    Article  PubMed  CAS  Google Scholar 

  9. Lowry, O.H., N.J. Rosenbrough, A.L. Farr, and R.J. Randall, J. Biol. Chem. 193:265 (1951).

    PubMed  CAS  Google Scholar 

  10. Hirsch, Y., and E.H. Ahrens, Jr., Ibid. 233:311 (1958).

    PubMed  CAS  Google Scholar 

  11. Björkhem, I., H. Danielsson, C. Issidorides, and A. Kallner, Acta Chem. Scand. 19:2151 (1965).

    PubMed  Google Scholar 

  12. Berséus, O., H. Danielsson, and A. Kallner, J. Biol. Chem. 240:2396 (1965).

    PubMed  Google Scholar 

  13. Shefer, S., S. Hauser, and E.H. Mosbach, J. Lipid Res. 9:328 (1968).

    PubMed  CAS  Google Scholar 

  14. Nicolau, G., S. Shefer, G. Salen, and E.H. Mosbach, Ibid. 15:146 (1974).

    CAS  Google Scholar 

  15. Shefer, S., G. Nicolau, and E.H. Mosbach, Ibid. J. Lipid Res. (In press).

  16. Einarsson, K., Eur. J. Biochem. 5:101 (1968).

    Article  PubMed  CAS  Google Scholar 

  17. Cohen, B.I., R.F. Raicht, S. Shefer, and E.H. Mosbach, Biochim. Biophys. Acta 369:79 (1974).

    PubMed  CAS  Google Scholar 

  18. Grundy, S.M., E.H. Ahrens, Jr., and T.A. Miettinen, J. Lipid Res. 6:397 (1965).

    PubMed  CAS  Google Scholar 

  19. Miettinen, T.A., E.H. Ahrens, Jr., and S.M. Grundy, Ibid. 6:411 (1965).

    PubMed  CAS  Google Scholar 

  20. Redinger, R.N., and D.M. Small, J. Clin. Invest. 52:161 (1973).

    Article  PubMed  CAS  Google Scholar 

  21. Lagarriga, J., and I.A.D. Bouchier, Gut 14:956 (1973).

    PubMed  CAS  Google Scholar 

  22. Berthelot, P., S. Erlinger, D. Dhumeaux, and A. Preaux, Amer. J. Physiol. 219:809 (1970).

    PubMed  CAS  Google Scholar 

  23. Mok, H.Y.L., P.M. Perry, and R.H. Dowling, Gut 15:247 (1974).

    PubMed  CAS  Google Scholar 

  24. Bernhardsson, C., I. Björkhem, H. Danielsson, and K. Wikvall, Biochem. Biophys. Res. Commun. 54:1030 (1973).

    Article  PubMed  CAS  Google Scholar 

  25. Björkhem, I., H. Danielsson, and J. Gustafsson, FEBS Lett. 31:20 (1973).

    Article  PubMed  Google Scholar 

  26. Strand, O., J. Lipid Res. 4:305 (1963).

    PubMed  CAS  Google Scholar 

  27. Paumgartner, G., W. Horak, P. Probst, and G. Grabner, Arch. Pharmak. 270:98 (1971).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Lipid Research, Public Health Research Institute of the City of New York, Inc., 10016, New York, New York

    B. I. Cohen

  2. Manhattan Veterans Administration Hospital, 10010, New York, New York

    R. F. Raicht

  3. Department of Lipid Research, Public Health Research Institute of the City of New York, Inc., 10016, New York, New York

    G. Nicolau & E. H. Mosbach

Authors
  1. B. I. Cohen
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  2. R. F. Raicht
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  3. G. Nicolau
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  4. E. H. Mosbach
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Cohen, B.I., Raicht, R.F., Nicolau, G. et al. Effects of phenobarbital upon bile acid synthesis in two strains of rats. Lipids 10, 168–174 (1975). https://doi.org/10.1007/BF02534155

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  • DOI: https://doi.org/10.1007/BF02534155

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