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Dealkylation of 24-ethylsterols byTetrahymena pyriformis

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Lipids

Abstract

WhenTetrahymena pyriformis was incubated with sitosterol ([24R]-24-ethylcholest-5-en-3β-ol]) or itstrans-Δ22-derivative (stigmasterol), the C-24-dealkylated product, cholesta-5,7,trans-22-trien-3β-ol, was obtained in both cases. 24(S)-24-Ethylcholesta-5,7,trans-22-trien-3β-ol also was found to be a metabolite. When sitosterol was the substrate, 24(R)-24-ethylcholesta-5,7-dien-3β-ol was obtained as a third product. Identifications were made by mass spectroscopy, quantitative chromatography, labeling with14C, and by other means. The dealkylated product (cholestratrienol) represented 30% of the sterols isolable after incubation. The administration of sterols to this organism did not induce sterol biosynthesis, since 2-14C-mevalonate failed to yield radioactive sterol in the presence of added stigmasterol.

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Authors and Affiliations

  1. Department of Biological Sciences, Drexel University, 19104, Philadelphia, Pennsylvania

    William R. Nes & Antonio Alcaide

  2. Departments of Chemistry and Biology, Bryn Mawr College, 19010, Bryn Mawr, Pennsylvania

    Frank B. Mallory, Josephine R. Landrey & Robert L. Conner

Authors
  1. William R. Nes
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  2. Antonio Alcaide
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  3. Frank B. Mallory
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  4. Josephine R. Landrey
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  5. Robert L. Conner
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Nes, W.R., Alcaide, A., Mallory, F.B. et al. Dealkylation of 24-ethylsterols byTetrahymena pyriformis . Lipids 10, 140–144 (1975). https://doi.org/10.1007/BF02534151

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  • DOI: https://doi.org/10.1007/BF02534151

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