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Metabolism of 2,4-3H-14α-methyl-5α-ergost-8-enol and 2,4-3H-5α-ergosta-8,14-dienol inChlorella ellipsoidea

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Lipids

Abstract

5α-Ergosta-8,14-dienol and 14α-methyl-5α-ergost-8-enol were synthesized chemically from ergosterol and labeled with tritium at the C-2 and C-4 positions. Both labeled sterols, when incubated with growing cultures ofChlorella ellipsoidea, were converted to ergost-5-enol but not to C-27 or C-29 sterols.Chlorella ellipsoidea, thus, has the capability of removing the C-14 methyl group and converting the 8(9) and 8,14 double bond systems to the 5(6) position. Brassicasterol produced by this organism is not labeled in these experiments, indicating that it is not derived from a saturated side chain precusor.

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Authors and Affiliations

  1. Botany Department, University of Maryland, 20742, College Park, Maryland

    L. B. Tsai & G. W. Patterson

  2. Insect Physiology Laboratory, Plant Protection Institute, 20705, Beltsville, Maryland

    C. F. Cohen

  3. Division of Biological and Medical Research, Argonne National Laboratory, 60439, Argonne, Illinois

    P. D. Klein

Authors
  1. L. B. Tsai
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  2. G. W. Patterson
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  3. C. F. Cohen
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  4. P. D. Klein
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Additional information

Scientific article no. A2020, contribution no. 4970 of the Maryland Agricultural Experiment Station.

ARS, USDA.

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Tsai, L.B., Patterson, G.W., Cohen, C.F. et al. Metabolism of 2,4-3H-14α-methyl-5α-ergost-8-enol and 2,4-3H-5α-ergosta-8,14-dienol inChlorella ellipsoidea . Lipids 9, 1014–1017 (1974). https://doi.org/10.1007/BF02533828

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  • DOI: https://doi.org/10.1007/BF02533828

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