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Accumulation of ergosta-8,14-dien-3β-ol bySaccharomyces cerevisiae cultured with an azasterol antimycotic agent

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Lipids

Abstract

15-Aza-24-methylene-D-homocholesta-8,14-dien-3β-ol, an antimycotic agent, at a concentration of 75 ng/ml inhibited ergosterol biosynthesis inSaccharomyces cerevisiae strain 3701 B resulting in the accumulation of an unusual sterol. Experimental data presented indicate that this sterol is ergosta-8,14-dien-3β-ol. The accumulation of the compound is supportive of current models of biosynthetic pathways for sterols in yeast and is consistent with inhibition by the azasterol of the Δ14 sterol reductase.

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Authors and Affiliations

  1. Department of Microbiology, Oregon State University, 97331, Corvallis, OR

    P. R. Hays & L. W. Parks

  2. Department of Chemistry, Simon Fraser University, V5A 1S6, Burnaby, B.C., Canada

    H. D. Pierce Jr. & A. C. Oehlschlager

Authors
  1. P. R. Hays
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  2. L. W. Parks
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  3. H. D. Pierce Jr.
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  4. A. C. Oehlschlager
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Hays, P.R., Parks, L.W., Pierce, H.D. et al. Accumulation of ergosta-8,14-dien-3β-ol bySaccharomyces cerevisiae cultured with an azasterol antimycotic agent. Lipids 12, 666–668 (1977). https://doi.org/10.1007/BF02533762

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  • DOI: https://doi.org/10.1007/BF02533762

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