Abstract
Lipids from bovine optic nerve were analyzed. The total content of 16.5% by weight included 27.2% nonpolar lipids, 26.1% glycolipids, and 46.7% phospholipids by weight. Free cholesterol was the major component of the nonpolar lipid fraction. The cerebrosides, 73.5% of total glycolipids, were separated by thin layer chromatography (TLC) into two bands (upper and lower) that were present in equal proportion. Cerebroside sulfates comprised about 27.5% of total glycolipids. Gangliosides were also detected in the glycolipid fraction. In order of predominance, choline glycerophospholipids, ethanolamine glycerophospholipids, ethanolamine plasmalogens, serine glycerophospholipids, sphingomyelins, and inositol glycerophospholipids were the major phospholipids. Palmitoyl (16∶0), stearoyl (18∶0), and oleoyl (18∶1) groups were the major acyl groups in all neutral and phospholipid classes. However, ethanolamine glycerophospholipids, serine glycerophospholipids, and inositol glycerophospholipids contained a large percentage of 22∶6 (docosahexaenoyl) group. The major alk-1-enyl groups of the plasmalogens were 16∶0, 18∶0, and 18∶1. Steroyl (18∶0), lignoceroyl (24∶0), and nervonoyl (24∶1) were the major acyl groups in all sphingolipids. Lower cerebroside band and cerebroside sulfates contained large amount of hydroxylignoceroyl (cerebronoyl) and hydroxynervonoyl groups.
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References
Horrocks, L.A., J. Lipid Res. 9:469 (1968).
Leitch, G.J., L.A. Horrocks, and T. Samorajski, J. Neurochem. 16:1347 (1969).
Banik, N.L., M.J. Blunt, and A.N. Davison, J. Neurochem. 15:471 (1968).
Bignani, A., and L.F. Eng, J. Neurochem. 20:165 (1973).
Detering, N.K., and M.A. Wells, J. Neurochem. 26:253 (1976).
Broekhuyse, R.M., Biochim. Biophys. Acta 152:307 (1968).
Anderson, R.E., and M.B. Maude, Arch. Biochim. Biophys. 151:270 (1971).
Urban, P.F., H. Dreyfus, N. Neskovic, and P. Mandel, J. Neurochem. 20:325 (1973).
Dorman, R.V., H. Dreyfus, L. Freysz, and L.A. Horrocks, Biochim. Biophys. Acta 486:55 (1977).
Fewsters, M.E., and J.F. Mead, J. Neurochem. 15:1041 (1968).
Galli, C., and R. Fumagalli, J. Neurochem. 15:35 (1968).
Wood, R., Lipids 9:429 (1974).
Berra, B., and V. Zambotti, Adv. Exp. Med. Biol. 25:311 (1972).
Folch, J., M. Lees, and G.A. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).
Wells, M.A., and J.C. Dittmer, Biochemistry 2:1259 (1963).
Bouser, G., G. Kritchevsky, and A. Yamamoto, in “Lipid Chromatographic Analysis,” Vol. 1, Dekker Inc., New York, 1967, p. 99.
Mangold, H.K., and D.C. Malins, J. Am. oil. Chem. Soc. 37:383 (1960).
Parker, F., and N.F. Peterson, J. Lipid Res. 6:455 (1965).
Rouser, F., S. Fleischer, and A. Yamamoto, Lipids 5:494 (1970).
Horrocks, L.A., J. Am. Oil Chem. Soc. 40:235 (1963).
Rouser, G., C. Galli, E. Leiber, M.L. Blank, and O.S. Privett, J. Am. Oil Chem. Soc. 41:836 (1964).
Siakotos, A.N., and G. Rouser, J. Am. Oil Chem. Soc. 42:913 (1965).
Rouser, G., A.N. Siakotos, and S. Fleischer, Lipids 1:85 (1966).
Dumphy, P.K., J. Whittle, and J.F. Pennock, Chem. Ind. (London) 1217 (1965).
Courchaine, A.J., W.H. Miller, and D.B. Stein, Jr., Clin. Chem. 5:609 (1959).
Van Handel, E., and D.B. Zilversmit, J. Lab. Clin. Med. 50:152 (1957).
Novak, M., J. Lipid Res. 6:431 (1965).
Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers, and F. Smith, Anal. Chem. 28:350 (1956).
Rosenberg, A., Biochemistry 2:1148 (1963).
Bartlett, G.R., J. Biol. Chem. 234:466 (1959).
Gottfried, E.L., and M.M. Rapport, J. Biol. Chem. 237:329 (1962).
Ferrell, W.J., J.F. Radloff, and A.B. Jackiw, Lipids 4:278 (1969).
M. Kates, in “Techniques of Lipidology,” American Elsevier Publishing Co., Inc., New York, 1972, p. 444.
Metcalf, L.D., and A.A. Schmitz, Anal. Chem. 33:363 (1961).
Das, S.K., M.T. Scott, and P.K. Adhikary, Lipids 10:584 (1975).
Sun, G.Y., and L.A. Horrocks, J. Lipid Res. 10:153 (1969).
Vance, D.E., and C.C. Sweeley, J. Lipid Res. 8:621 (1967).
Siddiqui, B., and R.H. McCluer, J. Lipid Res. 9:366 (1968).
Sweeley, C.C., R. Bentley, M. Makita, and W.W. Wells, J. Am. Chem. Soc. 85:2497 (1963).
Farquhar, J.W., W. Insull, P. Rosen, W. Stoffel, and E.H. Ahrens, Jr., Nutr. Rev. Suppl. 27:(8), Part 2:1 (1959).
Bazan, N.G., Jr., Biochim. Biophys. Acta 218:1 (1970).
Oulton, M., and C. Mezei, Lipids 8:235 (1973).
Organisciak, D.T., and J.D. Klingman, Lipids 9:307 (1974).
O'Brien, J.S., D.L. Fillerup, and J.F. Mead, J. Lipid Res. 5:109 (1964).
Holm, M., and J.E. Mansson, FEBS Lett. 45:159 (1974).
Landis, D.J., P.A. Dudley, and R.E. Anderson, Science 182:1144 (1973).
Svennerholm, L., J. Lipid Res. 9:570 (1968).
Sun, G.Y., J. Lipid Res. 14:656 (1973).
Eichberg, J., and R.M.C. Dawson, Biochem. J. 96:644 (1965).
Hokin, L.E., and M.R. Hokin, J. Gen. Physiol. 44:61 (1960).
Kennedy, E.P., in “The Neurosciences: A Study Program,” Edited by G.C. Quarton, T. Melnechuk, and F.O. Schmitt, Rockefeller Univ. Press, New York, 1967, p. 271.
Michell R.H., Biochim. Biophys. Acta 415:81 (1975).
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This investigation was supported by Grants DE-03191 and 2S06-RR-08037 from the National Institutes of Health, Bethesda, MD.
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Das, S.K., Steen, M.E., McCullough, M.S. et al. Composition of lipids of bovine optic nerve. Lipids 13, 679–684 (1978). https://doi.org/10.1007/BF02533745
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DOI: https://doi.org/10.1007/BF02533745