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Differences in the phospholipid, cholesterol, and fatty acyl composition of 3T3 and SV3T3 plasma membranes

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Lipids

Abstract

An analysis of the phospholipid, cholesterol, and phospholipid fatty acyl composition of isolated plasma membranes of 3T3 and SV3T3 mouse embryo cells has been performed. The results show that the plasma membrane of SV3T3 cells contain relatively less phosphatidylethanolamine and sphingomyelin and more cholesterol than 3T3 plasma membranes. The fatty acyl composition of individual phospholipid classes as determined by gas liquid chromatography also showed differences between 3T3 and SV3T3 plasma membranes. The plasma membranes of SV40 transformed 3T3 cells contain: (a) a higher percentage of 18∶1 and less 20∶3 and 20∶4 in phosphatidylethanolamine; (b) a higher percentage of 18∶1 in phosphatidylserine; and (c) a higher percentage of 18∶2 and 20∶4 in phosphatidylinositol.

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References

  1. Wallach, D.F.H., in “Membrane Molecular Biology of Neoplastic Cells,” Elsevier Scientific Publishing Co., New York, 1975.

    Google Scholar 

  2. Hynes, R.O., Cell 1:147 (1975).

    Article  Google Scholar 

  3. van Nest, G.A., and W.J. Grimes, Biochemistry 16:2902 (1977).

    Article  PubMed  Google Scholar 

  4. Hakomori, S.T., in “Tumor Lipids: Biochemistry and Metabolism,” American Oil Chemists' Society Press, Champaign, IL, 1973, p. 269.

    Google Scholar 

  5. Furcht, L.T., R.E. Scott, and P.B. Maercklein, Cancer Res. 36:4584 (1977).

    Google Scholar 

  6. Scott, R.E., P.B. Maercklein, and L.T. Furcht, J. Cell Sci. 344:263 (1977).

    Google Scholar 

  7. Plagemann, P.G.W., and D.P. Richey, Biochim. Biophys. Acta 344:263 (1974).

    PubMed  CAS  Google Scholar 

  8. Ryan, W.L. and M.L. Heidrick, Adv. Cyclin Nucleotide Res. 8:81 (1974).

    Google Scholar 

  9. Burger, M.M., and A.R. Goldberg, Proc. Natl. Acad. Sci. USA 57:359 (1967).

    Article  PubMed  CAS  Google Scholar 

  10. Horwitz, A.L., M.E. Hatten, and M.M. Burger, Proc. Natl. Acad. Sci. USA 71:3115 (1974).

    Article  PubMed  CAS  Google Scholar 

  11. Fuchs, P., A. Parola, P.W. Robbins, and E.R. Blout, Proc. Natl. Acad. Sci. USA 72:3115 (1974).

    Google Scholar 

  12. Inbar, M., I. Yuli and A. Raz, Exp. Cell Res. 105:325 (1977).

    Article  PubMed  CAS  Google Scholar 

  13. Scott, R.E., Science 194:743 (1976).

    Article  PubMed  CAS  Google Scholar 

  14. Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall, J. Biol. Chem. 193:265 (1951).

    PubMed  CAS  Google Scholar 

  15. Bohlen, P., S. Stein, W. Dairman, S. Udenfriend, Arch. Biochem. Biophys. 155:213 (1973).

    Article  PubMed  CAS  Google Scholar 

  16. Knox, F.G., J. Preiso, J.K. Kim, and T.P. Dousa, J. Clin. Invest. 59:675 (1977).

    Article  PubMed  CAS  Google Scholar 

  17. Perkins, R.G. and F.A. Kummerow, Biochim. Biophys. Acta 424:469 (1976).

    PubMed  CAS  Google Scholar 

  18. Searcy, R.L., and L.M. Berguist, Clin. Chim. Acta 5:192 (1960).

    Article  PubMed  CAS  Google Scholar 

  19. Eng, L.F., and E.P. Noble, Lipids 3:157 (1968).

    Article  CAS  PubMed  Google Scholar 

  20. Johnston, P.V., in “Basic Lipid Methodology,” Univ. of Ill. Press, Urbana-Champaign, IL, 1971, p. 46.

    Google Scholar 

  21. Shinitzky, M., and M. Inbar, Biochim. Biophys. Acta 433:133 (1976).

    Article  PubMed  CAS  Google Scholar 

  22. Micklem, K.J., R.M. Abra, S. Knutton, J.M. Graham and C.A. Pasternak, Biochem. J. 154:561 (1976).

    PubMed  CAS  Google Scholar 

  23. van Hoeven, R.P., and P. Emmelot, J. Membr. Biol. 9:105 (1972).

    Article  PubMed  Google Scholar 

  24. van Hoeven, R.P., P. Emmelot, J.H. Krol, and E.P.M. Omen-Meulemans, Biochim. Biophys. Acta 380:1 (1975).

    PubMed  Google Scholar 

  25. Ruggieri, S., and A.T. Fallani, in “Tumor Lipids: Biochemistry and Metabolism,” American Oil Chemists' Society Press, Champaign, IL, 1975, p. 89.

    Google Scholar 

  26. Bergelson, L.D., and E.V. Dyatlovitskaya, in “Tumor Lipids: Biochemistry and Metabolism,” American Oil Chemists' Society Press, Champaign, IL 1975, p. 111.

    Google Scholar 

  27. Howard, B.V., J.D. Butler and J.M. Bailey, in “Tumor Lipids: Biochemistry and Metabolism,” American Oil Chemists' Society Press, Champaign, IL, 1975, p. 200.

    Google Scholar 

  28. Brivio-Haugland, R.P., S.L. Louis, K. Musch, N. Waldeck, and M.A. Williams, Biochim. Biophys. Acta 433:150 (1976).

    Article  PubMed  CAS  Google Scholar 

  29. Ritzi, E.M., and W.A. Stylos, J. Natl. Cancer Inst. 56:529 (1976).

    PubMed  CAS  Google Scholar 

  30. Waite, M., B. Parce, R. Morton, C. Cunningham, and H.P. Morris, Cancer Res. 37:2092 (1977).

    PubMed  CAS  Google Scholar 

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Author notes

  1. Robert E. Scott

    Present address: Laboratory of Membrane Pathology, Department of Pathology & Anatomy, Mayo Clinic/Foundation, 55901, Rochester, Minnesota

  2. Roger G. Perkins

    Present address: 3M Company, 3M Center Bldg. 220-2E, 55101, St. Paul, MN

Authors and Affiliations

  1. Laboratory of Membrane Pathology, Department of Pathology & Anatomy, Mayo Clinic/Foundation, 55901, Rochester, Minnesota

    Roger G. Perkins

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  1. Roger G. Perkins
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  2. Robert E. Scott
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Perkins, R.G., Scott, R.E. Differences in the phospholipid, cholesterol, and fatty acyl composition of 3T3 and SV3T3 plasma membranes. Lipids 13, 653–657 (1978). https://doi.org/10.1007/BF02533741

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  • DOI: https://doi.org/10.1007/BF02533741

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