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Biosynthesis of unsaturated fatty acids in the diatomPhaeodactylum tricornutum

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Lipids

Abstract

The biosynthesis of fatty acids in the diatomPhaeodactylum tricornutum was studied. The diatom was incubated with sodium [114C] acetate and the acids [1-14C] palmitic, [1-14C] stearic, [1-14C] linoleic and [1-14C] α-linolenic. The distribution of radioactivity in the products was determined by gas liquid radiochromatography. The diatom synthesized “de novo” not only saturated and monounsaturated fatty acids, but also linoleic, α-linolenic and other fatty acids including the highly polyunsaturated 20∶5ω3 and 22∶6ω3. When labeled acetate, stearic, α-linolenic or even linoleic acid were incubated with the diatom, the polyunsaturated C20 fatty acids synthesized belonged predominantly to the ω 3 family. The existence of Δ9, Δ6, Δ5, Δ4, ω6 and possibly ω3 desaturases inP. tricornutum is suggested.

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Author notes

  1. Victor J. Moreno & Julia E. A. de Moreno

    Present address: Instituto Nacional de Investigación y Desarrollo Pesquero, Casilla de Correo 175, 7600, Mar del Plata, Argentina

Authors and Affiliations

  1. Cátedra de Química Biológica, Dpto. de Biología, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina

    Victor J. Moreno, Julia E. A. de Moreno & Rodolfo R. Brenner

  2. I.N.I.D.E.P., Argentina

    Victor J. Moreno, Julia E. A. de Moreno & Rodolfo R. Brenner

  3. Cátedra de Bioquímica, Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina

    Victor J. Moreno, Julia E. A. de Moreno & Rodolfo R. Brenner

Authors
  1. Victor J. Moreno
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  2. Julia E. A. de Moreno
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  3. Rodolfo R. Brenner
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Member of the Carrera del Investigador Científico of the Comisión de Investigaciones Científicas de la Provincia de Buenos Aires.

Member of the Carrera del Investigador Cientifico of the Consejo Nacional de Investigaciones Cientificas y Técnicas.

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Moreno, V.J., de Moreno, J.E.A. & Brenner, R.R. Biosynthesis of unsaturated fatty acids in the diatomPhaeodactylum tricornutum . Lipids 14, 15–19 (1979). https://doi.org/10.1007/BF02533560

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  • DOI: https://doi.org/10.1007/BF02533560

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