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Synthesis and characterization of [1-13C]-and d8-arachidonic acid

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Lipids

Abstract

Methyld 8- and [1-13C] 5,8,11,14-eicosatetraenoate (arachidonate) were prepared from a common synthetic precursor, 4,7,10,13-nonadecatetrayn-1-ol. The purified products were characterized by gas chromatography-mass spectrometry. Mass spectra oft-butyldimethylsilyl esters ofd 8-and [1-13C]-arachidonic acid showed a most intense [M-57]+ peak at high mass. The isotopic purity of methyl [1-13C] arachidonate was 99% and that of methyld 8-arachidonate was 56%. Whend 8-arachidonic acid was prepared by direct deuteration of 5,8,11,14-eicosatetraynoic acid, the isotopic purity of the sample was 86%.

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Authors and Affiliations

  1. Applied Science Division, Milton Roy Company, 16801, State College, Pennsylvania

    Un Hoi Do, M. G. Sundaram & S. Ramachandran

  2. Department of Biochemistry, School of Medicine and Health Sciences, George, Washington University, 20037, Washington, DC

    Robert W. Bryant

Authors
  1. Un Hoi Do
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  2. M. G. Sundaram
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  3. S. Ramachandran
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  4. Robert W. Bryant
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Do, U.H., Sundaram, M.G., Ramachandran, S. et al. Synthesis and characterization of [1-13C]-and d8-arachidonic acid. Lipids 14, 819–821 (1979). https://doi.org/10.1007/BF02533522

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  • DOI: https://doi.org/10.1007/BF02533522

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