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End product specificity of triacylglycerol lipases from intestine, fat body, muscle and haemolymph of the American cockroach,Periplaneta americana L.

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Lipids

Abstract

The triacylglycerol-hydrolyzing capacity of tissue homogenates has been investigated for midgut, fat body, thoracic musculature and haemolymph of the American cockroach,Periplaneta americana. The greatest lipolytic activity was demonstrated in midgut homogenates with decreasing levels of activity present in fat body, muscle and haemolymph. Comparison of the lipolytic products resulting from triacylglycerol hydrolysis indicates that midgut homogenates effect the production ofsn-2-monoacylglycerols and free fatty acids, whereas the other tissues that were examined favor the accumulation of diacylglycerols. Stereospecific analysis of the diacylglycerol products of triacylglycerol hydrolysis demonstrated that the lipolytic activities of midgut and muscle homogenates result in the production of a racemic mixture of thesn-1,2-andsn-2,3-enantiomers, but the fat body and haemolymph show a preference for the accumulation of thesn-1,2-isomer.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research, C.H. Best Institute, University of Toronto, M5G 1L6, Toronto, Ontario, Canada

    Alan G. D. Hoffman

  2. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    R. G. H. Downer

Authors
  1. Alan G. D. Hoffman
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  2. R. G. H. Downer
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Hoffman, A.G.D., Downer, R.G.H. End product specificity of triacylglycerol lipases from intestine, fat body, muscle and haemolymph of the American cockroach,Periplaneta americana L.. Lipids 14, 893–899 (1979). https://doi.org/10.1007/BF02533502

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  • DOI: https://doi.org/10.1007/BF02533502

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