Abstract
Mixed brain gangliosides or individual ganglioside GM1 were dissolved in one of the following solvents: (a) water, (b) 0.1 M aqueous KC1, or (c) methanol-aqueous 0.1 M KC1 at concentrations ranging from 10−8 to 10−3 M, and were submitted to dialysis against distilled water for up to 4 days. No significant loss of gangliosides on dialysis was observed when gangliosides were dissolved in water or 0.1 M aqueous KC1, but a loss occurred when the ganglioside solution contained methanol; the loss was greater at the lowest ganglioside concentration. However, losses ceased to occur after 1 day when methanol was removed from the dialysis sac.
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References
Folch-Pi, J., M. Lees, G.H. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).
Suzuki, K., J. Neurochem. 12:629 (1965).
Tettamanti, G., F. Bonali, S. Marchesini, and V. Zambotti, Biochim. Biophys. Acta 296:160 (1973).
Kanfer, J., and C. Spielvogel, J. Neurochem. 20:1483 (1973).
Dunn, A., J. Neurochem. 23:293 (1974).
Faryna de Raveglia, I., and N.E. Ghittoni, J. Chromatogr. 58:288 (1971).
Svennerholm, L., in “Comprehensive Biochemistry,” Vol. 18, Edited by M. Florkin and E.H. Stotz, Pergamon Press, Oxford, 1970, p. 201.
Suzuki, Y., and K. Suzuki, J. Lipid Res. 13:687 (1972).
Ghidoni, R., G. Tettamanti, and V. Zambotti, Ital. J. Biochem. 23:101 (1974).
Svennerholm, L., Biochim. Biophys. Acta 24:604 (1957).
Wiegandt, H., Adv. Lipid Res. 9:249 (1971).
Heuser, E., K. Lipp, and H. Wiegandt, Anal. Biochem. 60:382 (1974).
Gammack, D.B., Biochem. J. 38:373 (1963).
Howard, D.E., and R.M. Burton, Biochim. Biophys, Acta 84:435 (1964).
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Ghidoni, R., Sonnino, S. & Tettamanti, G. Behavior of gangliosides on dialysis. Lipids 13, 820–822 (1978). https://doi.org/10.1007/BF02533483
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DOI: https://doi.org/10.1007/BF02533483