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Isolation and identification of the metabolites of 22,25-dideoxyecdysone from cockroach fat body cultures

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Lipids

An Erratum to this article was published on 01 December 1978

Abstract

Hydroxylation and conjugation were the principal pathways of metabolism of 22,25-dideoxyecdysone in cockroach fat body cultures. The major metabolite isolated and identified was the tetrahydroxy steroid 22-deoxyecdysone; other exdysteroids isolated, in order of decreasing quantities, were 22-deoxy-26-hydroxyecdysone, 22,25-dideoxy-26-hydroxyecdysone, and 22-deoxy-20-hydroxyecdysone. Cockroach fat body from late-instar nymphs appears to lack the mechanism for hydroxylating at C-22. Radioanalyses of the material obtained from enzymic hydrolysis of the conjugate fraction showed 65, 15 and 20% of tetraols, pentaols, and unhydrolyzed conjugates respectively, and no 22,25-dideoxyecdysone. An azasteroid and two nonsteroidal amines that effectively inhibit the activity of 22,25-dideoxyecdysone in the cockroach leg regenerate-fat body culture system enhanced the metabolism of 22,25-dideoxyecdysone, decreased the quantity of the pentaol fraction present, and caused an increase or accumulation of the tetraol and conjugate fractions in the fat body culture system.

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Author notes

  1. Edwin P. Marks & Patricia A. Filipi

    Present address: Metabolism and Radiation Research Laboratory, Federal Research, Science and Education Administration, U.S. Department of Agriculture, 58102, Fargo, North Dakota

  2. Harold Finegold

    Present address: Analytical Chemistry Laboratory, Federal Research, Science and Education Administration, U.S. Department of Agriculture, 20705, Beltsville, Maryland

Authors and Affiliations

  1. Insect Physiology Laboratory, Federal Research, Science and Education Administration, U.S. Department of Agriculture, 20705, Beltsville, Maryland

    Malcolm J. Thompson, William E. Robbins & Samson R. Dutky

Authors
  1. Malcolm J. Thompson
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  2. William E. Robbins
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  3. Samson R. Dutky
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  4. Edwin P. Marks
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  5. Patricia A. Filipi
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02533835.

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Thompson, M.J., Robbins, W.E., Dutky, S.R. et al. Isolation and identification of the metabolites of 22,25-dideoxyecdysone from cockroach fat body cultures. Lipids 13, 783–790 (1978). https://doi.org/10.1007/BF02533476

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  • DOI: https://doi.org/10.1007/BF02533476

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