Skip to main content
SpringerLink
Log in

Simultaneous mass spectrometric measurement of prostaglandins E1 (PGE1) and PGE2 with a deuterated internal standard

  • Methods
  • Published:
Lipids

Abstract

The use of (3,3,4,4-2H4)PGE2 as internal standard for the quantitative measurement of PGE1 by gas chromatography-mass spectrometry is reported. A method for the simultaneous analysis of PGE1 and PGE2 is also described. The prostaglandins were analyzed by selected ion monitoring as the methyl ester-trimethylsilyl ether (ME-TMS) derivatives of PGB1 and PGB2, respectively. In all cases, a linear response over the range 1 to 70 ng (2.8 to 198 pmoles) was demonstrated. The use of this procedure is exemplified by its application to the PG analysis of sheep seminal vesicles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. McCosh, E.J., D.L. Meyer, and J. Dupont, Prostaglandins 12:471 (1976).

    Article  PubMed  CAS  Google Scholar 

  2. Van Orden, D.E., D.B. Farley, and C.J. Clancey, Prostaglandins 13:437 (1977).

    Article  PubMed  Google Scholar 

  3. Samuelsson, B., M. Hamberg, and C.C. Sweeley, Anal. Biochem. 38:301 (1970).

    Article  PubMed  CAS  Google Scholar 

  4. Axen, U., K. Gréen, D. Hörlin, and B. Samuelsson, Biochem. Biophys. Res. Commun. 45:519 (1971).

    Article  PubMed  CAS  Google Scholar 

  5. Gréen, K., E. Granström, B. Samuelsson, and U. Axen, Anal. Biochem. 54:434 (1973).

    Article  PubMed  Google Scholar 

  6. Axen, U., L. Baczynskyj, D.J. Duchamp, K.T. Kirton, and J.F. Zieserl, Jr., in “Advances in the Biosciences,” Vol. 9, Edited by S. Bergström, Pergamon Press, New York, 1973, p. 109.

    Google Scholar 

  7. Sweetman, B.J., J.C. Frölich, and J.T. Watson, Prostaglandins 3:75 (1973).

    Article  PubMed  CAS  Google Scholar 

  8. Kelly, R.W., Anal. Chem. 45:2079 (1973).

    Article  PubMed  CAS  Google Scholar 

  9. Middleditch, B.S., and D.M. Desiderio, Prostaglandins 4:459 (1973).

    Article  PubMed  CAS  Google Scholar 

  10. Nicosia, S., and G. Galli, Anal. Biochem. 61:192 (1974).

    Article  PubMed  CAS  Google Scholar 

  11. Vane, F., and M.G. Horning, Anal. Lett. 2:357 (1969).

    CAS  Google Scholar 

  12. Gréen, K., Chem. Phys. Lipids 3:254 (1969).

    Article  PubMed  Google Scholar 

  13. Perry, D.L., and D.M. Desiderio, Prostaglandins 14:745 (1977).

    Article  PubMed  CAS  Google Scholar 

  14. Beynon, J.H., “Mass Spectrometry and Its Applications to Organic Chemistry, Chapter 10, Elsevier, New York, 1960, p. 429.

    Google Scholar 

  15. Murphy, R.C., J.A. Zirrolli, K. Clay, S.E. Hattox, and R. Helbig, Proc. 23rd Ann. Confer. on Mass Spec. and Allied Topics, p. 427 (1975).

Download references

Author information

Authors and Affiliations

  1. Lipid Nutrition Laboratory, Nutrition Institute, Human Nutrition Center, Science and Education Administration, U.S. Department of Agriculture, 20705, Beltsville, Maryland

    Aldo Ferretti & Vincent P. Flanagan

Authors
  1. Aldo Ferretti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vincent P. Flanagan
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable.

About this article

Cite this article

Ferretti, A., Flanagan, V.P. Simultaneous mass spectrometric measurement of prostaglandins E1 (PGE1) and PGE2 with a deuterated internal standard. Lipids 14, 483–491 (1979). https://doi.org/10.1007/BF02533466

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02533466

Keywords

Search

Navigation