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The effect of steroids and their solubilizing agents on mycelial growth ofPhytophthora cactorum

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Lipids

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Abstract

Mycelia ofPhytophthora cactorum, a fungus unable to synthesize sterols, were incubated with a series of sterols and several common sterol solubilizing agents. Cholesterol, campesterol, and sitosterol promoted growth, while carpesterol, saringosterol, 20 α-hydroxy cholesterol, coprostanol, and 5α-cholestane inhibited growth. Cholestanol, epicholestanol, and 5α-cholestan-3-one had no significant effect on growth relative to the sterol-free control. All steroids were solubilized in ethanol for studying structure-activity correlations. The added steroids were reisolated from mycelia and their identity confirmed by gas liquid chromatography (GLC) or combined gas liquid chromatography-mass spectrometry (GLC-MS). Tween 80 promoted growth, Triton X-100 inhibited growth, and ethanol had no effect on growth relative to a control.

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Authors and Affiliations

  1. Department of Botany, University of Maryland, 20742, College Park, MD

    W. David Nes, Glenn W. Patterson & George A. Bean

Authors
  1. W. David Nes
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  2. Glenn W. Patterson
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  3. George A. Bean
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Additional information

Scientific Article No. A2441, Contribution No. 5469 of the Maryland Agricultural Experiment Station.

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Nes, W.D., Patterson, G.W. & Bean, G.A. The effect of steroids and their solubilizing agents on mycelial growth ofPhytophthora cactorum . Lipids 14, 458–462 (1979). https://doi.org/10.1007/BF02533462

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  • DOI: https://doi.org/10.1007/BF02533462

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