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A simplified colorimetric micromethod for determination of serum cholesterol

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Lipids

Abstract

A simple and rapid micromethod is described for quantitation of cholesterol in 5–10 μl of serum using uranyl acetate to deproteinize the serum ando-phthalaldehyde reagent to develop color. Independent analyses of serum samples by the present method and by two reference methods, Abell-Kendall and automated colorimetric method, indicated accuracy of the micromethod (r=0.99). Use of capillary blood specimens makes this procedure applicable for screening infants and small children.

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References

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Authors and Affiliations

  1. Department of Medicine, Louisiana State University Medical Center, New Orleans, LA

    Rao R. Bhandaru, Sathanur R. Srinivasan, Padmakar S. Pargaonkar & Gerald S. Berenson

Authors
  1. Rao R. Bhandaru
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  2. Sathanur R. Srinivasan
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  3. Padmakar S. Pargaonkar
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  4. Gerald S. Berenson
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Bhandaru, R.R., Srinivasan, S.R., Pargaonkar, P.S. et al. A simplified colorimetric micromethod for determination of serum cholesterol. Lipids 12, 1078–1080 (1977). https://doi.org/10.1007/BF02533337

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  • DOI: https://doi.org/10.1007/BF02533337

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