Abstract
The body-wall-outgrowth response of the rotiferAsplanchna sieboldi to tocopherol has been developed into a new bioassay which can estimate tocopherol titres more than 106 times more dilute than the detection limits of previously reported bioassays, and which is more sensitive and permits greaters qualitative discrimination than any fluorometric assay. this rotifer bioassay may prove especially useful in determining biological activities of tocopherol compounds and in estimating total tocopherol activity in biological material of very limited size or tocopherol content. It may also be used to analyze and describe the effects of environmental, genetic, and pharmacological factors on the body-wall-out-growth responses ofAsplanchna to given doses of tocopherol in terms of regression equations or equivalent changes in inducing tocopherol concentrations.
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This work was done in partial fulfillment of the requirements for the Ph.D. at Dartmouth College.
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Kabay, M.E., Gilbert, J.J. A new rotifer-based assay for tocopherol. Lipids 12, 875–878 (1977). https://doi.org/10.1007/BF02533280
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DOI: https://doi.org/10.1007/BF02533280