Triglyceride sub-classes of dog plasma and organs

Abstract

Lipids were extracted from liver, kidney, myocardium, skeletal muscle, arterial and femoral venous plasma. Triglycerides were purified by thin layer chromatography and the double bond subclasses separated by silver nitrate silica gel H thin layer plates. Gas chromatography was used to delineate the fatty acid composition of each sub-class as well as the carbon number of the purified triglyceride from each site. The major double bond sub-class was O11 (O,saturated fatty acid; 1,one double bond; 2,two double bonds). The plasma values were highest in 001 and 002. All sites had quite uniform 112 while plasma had the lowest 012. The percentage of all saturated fatty acids in a band was summed and equated to 100% and the fatty acid percentage recalculated on this basis. The saturated fatty acid distribution in myocardium and skeletal muscle was highest in myristate in the third 001 sub-class and liver and plasma had the highest stearate in the second 001 band. The palmitate was similar in most bands. Every sample, except the subcutaneous adipose tissue, had a maximum triglyceride content of carbon number 52 (the carbon number represented the sum of all carbon atoms in the fatty acids present in a molecule). The liver and subcutaneous adipose tissue triglyceride carbon number 52 and 54 were approximately equal. The liver exhibited the largest 56.