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Quantitative analysis of free and bound fatty aldehydes: Optimum conditions for p-nitrophenylhydrazone formation

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Lipids

Abstract

A method is described for the analysis of free and bound fatty aldehydes in lipid extracts. By radiolabeling techniques it was shown that the method for measuring free fatty aldehydes when used in the presence of bound fatty aldehydes measures less than 1% of the bound aldehydes. Parameters of time, temperature and optimum acid concentration are reported. A comparison has been made between the present method and other published methods of measuring p-nitrophenylhydrazones with emphasis on recoveries and reactivities. The present method is suitable for measuring as little as 0.02 μmoles of fatty aldehyde. The method has been applied to the analysis of free and bound aldehydes present in various mouse tissues.

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Authors and Affiliations

  1. Department of Chemistry, Biochemistry Division, University of Detroit, 48221, Detroit, Michigan

    William J. Ferrell, James F. Radloff & Andrew B. Jackiw

Authors
  1. William J. Ferrell
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  2. James F. Radloff
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  3. Andrew B. Jackiw
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Ferrell, W.J., Radloff, J.F. & Jackiw, A.B. Quantitative analysis of free and bound fatty aldehydes: Optimum conditions for p-nitrophenylhydrazone formation. Lipids 4, 278–282 (1969). https://doi.org/10.1007/BF02533186

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  • DOI: https://doi.org/10.1007/BF02533186

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