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Kainic acid, AMPA, and dihydrokainic acid effect on uptake and efflux ofd-[3H]aspartic acid in cerebellar slices

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Abstract

In this study we show that the glutamate ionotropic agonist kainate (KA) stimulates the efflux of preloadedd-[3H]aspartate (D-[3H]Asp) and inhibits the uptake of this amino acid in cerebellar slices. The effect of this agonist on the efflux of D-[3H]Asp is sensitive to(i) 6-nitro-7-sulphamoylbenzo(f)quinoxaline-2-3-dione (NBQX), indicating the involvement of KA/(RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, and is(ii) partially tetrodotoxin (TTX)-sensitive, indicating that pre-(TTX-insensitive) and post-synaptic (TTX-sensitive) KA/AMPA receptors are involved. In contrast, the effect on uptake is NBQX- and TTX-insensitive indicating a direct interaction with glutamate transporters. AMPA inhibited D-[3H]Asp uptake and had no effect on D-[3H]Asp efflux. In the same system, the uptake but not the efflux of D-[3H]Asp was affected by dihydrokainate (DHK). The DHK-induced uptake inhibition occurred in the presence of TTX. NBQX inhibited DHK-induced effect at 5 mM but not at 1 mM DHK concentrations.

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Authors and Affiliations

  1. Laboratoire de Neurobiologie Cellulaire, UPR 9009 CNRS, Centre de Neurochimie, 5, rue Blaise Pascal, F-67084, Strasbourg, France

    M. Bouazzaoui, C. Kannengieser, O. Procksch & G. Gombos

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  1. M. Bouazzaoui
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  2. C. Kannengieser
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  3. O. Procksch
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  4. G. Gombos
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Bouazzaoui, M., Kannengieser, C., Procksch, O. et al. Kainic acid, AMPA, and dihydrokainic acid effect on uptake and efflux ofd-[3H]aspartic acid in cerebellar slices. Neurochem Res 21, 1527–1533 (1996). https://doi.org/10.1007/BF02533101

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