Abstract
The effect of temperature on the lipid composition ofCephalosporium falciforme andCephalosporium kiliense, causative agents of maduromycosis, was investigated. The fungi were grown at 28.5 C and 37 C in a chemically defined medium. The lipids were solvent extracted, purified on Sephadex, and separated into their component classes by silicic acid column chromatography. Five lipid classes were found: (a) sterol esters, (b) triacylglycerides, (c) free fatty acids, (d) sterols, and (e) phospholipids. Fatty acids were analyzed by gas liquid chromatography and phospholipids by thin layer chromatography. Temperature induced changes of varying degrees occurred in both the fatty acid phospholipid fractions of each organism.
References
Weete, J.D., “Fungal Lipid Biochemistry,” Plenum Press, New York, NY, 1974.
Farrell, J., and A.H. Rose, Ann. Rev. Microbiol. 21:101 (1967).
Peck, R.L., JAOCS 64:487 (1942).
Nickerson, W.J., “Biology of Pathogenic Fungi,” Chronica Botanica Co, Waltham, MA, 1947.
Combs, T.J., J.J. Guarneri, and M.A. Pisano, Mycologia 60:1232 (1968).
Huston, C.K., and P.W. Albro, J. Bact. 88:425 (1964).
Siakotos, A.N., and G. Rouser, JAOCS 42:913 (1965).
Barron, E.J., and D.J. Hanahan, J. Biol. Chem. 231:493 (1958).
James, A.T., “Methods of Biochemical Analysis,” Interscience Publishers, New York, NY, 1959.
Metcalfe, L.E., and A.A. Schmitz, Anal. Chem. 33:363 (1961).
Rouser, G., A.N. Siakotos, and S. Fleischer, Lipids 1:85 (1966).
Bartlett, G.R., J. Biol. Chem. 234:466 (1959).
Ghoshal, J., Sci. Cult. 35:694 (1969).
Aronson, S., and B. Volh, “Cerebral Sphingolipidoses,” Academic Press, New York, NY, 1962.
Parmegiani, R.M., and M.A. Pisano, Develop. Ind. Microbiol. 15:318 (1974).
Collins, R., and K. Kalnins, Mycopath. Mycol. Appl. 36:214 (1968).
Epstein, W.W., E. Aoyagi, and P.W. Jennings, Comp. Biochem. Physiol. 18:225 (1966).
Weiss, B., and R. Stiller, Biochemistry 11:4522 (1972).
DiSalvo, A.F., and G.F. Denton, J. Bact. 85:927 (1963).
Author information
Authors and Affiliations
About this article
Cite this article
Sawicki, E.H., Pisano, M.A. The effect of temperature on the fatty acid and phospholipid composition ofCephalosporium falciforme andCephalosporium kiliense . Lipids 12, 125–127 (1977). https://doi.org/10.1007/BF02532984
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02532984