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Study of bound phospholipase activities of fungal mycelia using an organic solvent system

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Lipids

Abstract

It has been possible to demonstrate and characterize high phospholipase activities in mycelia ofRhizopus arrhizus andMucor javanicus by use of a system in which substrates were dissolved in diisopropyl ether. Such activities were associated with bound enzymes and would have been difficult to detect using aqueous assay systems. In both cases, phosphatidylcholine hydrolysis was by phospholipase A1 (EC 3.1.1.32) activity followed by the action of lysophospholipase (EC 3.1.1.5). Phospholipase D (EC 3.1.4.4) activity was also detected. The methods used appear to be of general applicability for the detection and study of insoluble phospholipases.

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Authors and Affiliations

  1. Biochemistry Department, University of Strathclyde, Biosciences Building, 31 Taylor Street, G4 0NR, Glasgow, Scotland

    J. A. Blain, J. D. E. Patterson, C. E. Shaw & M. Waheed Akhtar

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  1. J. A. Blain
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  2. J. D. E. Patterson
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  3. C. E. Shaw
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  4. M. Waheed Akhtar
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Blain, J.A., Patterson, J.D.E., Shaw, C.E. et al. Study of bound phospholipase activities of fungal mycelia using an organic solvent system. Lipids 11, 553–560 (1976). https://doi.org/10.1007/BF02532901

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  • DOI: https://doi.org/10.1007/BF02532901

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