Abstract
The contents of phosphatidyl N-methyl-and N,N-dimethylaminoethanol were determined in the liver of rats injected with (Me-C14) methionine. Total phospholipids were extracted from aliquots of the liver and fractionated by two-dimensional thin layer chromatography after addition of carrier phosphatidyl-N-methyl-and N,N-dimethylaminoethanol. The radioactivity present in the two phosphatide spots was determined and used to calculated total disintegrations per min/100 g body wt. The remainder of the livers was pooled, and total phospholipids were isolated and subjected to acid hydrolysis. N-methyl- and N,N-dimethylaminoethanol were purified by thin layer chromatography, and their specific activity was determined after quantitation by gas liquid chromatography and radioactivity measurement. The liver contents of phosphatidyl N-methyl- and N,N-dimethylaminoethanol were determined by dividing disintegrations per min/100 g body wt by the specific activity of N-methyl- or N,N-dimethylaminoethanol.
Similar content being viewed by others
References
Katyal, S.L., and B. Lombardi, Lipids 9:81 (1974).
Lester, R.L., and D.C. White, J. Lipid Res. 8:565 (1967).
Bremer, J., P.H. Figard, and D.M. Greenberg, Biochim. Biophys. Acta 43:477 (1960).
Lombardi, B., and A. Oler, Lab. Invest. 17:308 (1967).
Bligh, E.G., and W.J. Dyer, Can. J. Biochem. Physiol. 37:911 (1959).
Arvidson, G.A.E., Eur. J. Biochem. 4:478 (1968).
Shin, Y.S., Anal. Chem. 34:1164 (1962).
Lombardi, B., and G. Ugazio, J. Lipid Res. 6:498 (1965).
Author information
Authors and Affiliations
About this article
Cite this article
Katyal, S.L., Lombard, B. Quantitation of phosphatidyl N-methyl- and N,N-Dimethylaminoethanol in rat liver. Lipids 11, 513–516 (1976). https://doi.org/10.1007/BF02532895
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02532895