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Methodology for separation of gangliosides from potential water-soluble precursors

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Lipids

Abstract

The following methods were examined for their ability to remove labeled nucleotide sugars and other water-soluble precursors from ganglioside preparations: enzymatic digestion of nucleotide sugars followed by dialysis; silicic acid and Sephadx G-25 column chromatography; ascending paper chromatography; high voltage paper electrophoresis; thin layer chromatography; co-precipitation of gangliosides and bovine serum albumin with trichloroacetic acid. A procedure was developed which allows these complex glycosphingolipids to remain in the chloroform-rich lower phase during conventional Folch partitioning.

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Authors and Affiliations

  1. Eunice Kennedy Shriver Center at the Walter E. Fernald State School, 200 Trapelo Road, 02154, Waltham, Massachussets

    T. P. Carter

  2. Neurology Research, Massachusetts General Hospital, 02114, Boston, Massachusetts

    J. Kanfer

Authors
  1. T. P. Carter
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  2. J. Kanfer
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Carter, T.P., Kanfer, J. Methodology for separation of gangliosides from potential water-soluble precursors. Lipids 8, 537–548 (1973). https://doi.org/10.1007/BF02532709

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  • DOI: https://doi.org/10.1007/BF02532709

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