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Effect of dietary cholesterol on bile-acid composition of gall bladder bile from guinea pigs

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Lipids

Abstract

The composition of gall bladder bile acids from control and cholesterol-fed, anemic guinea pigs was analyzed by thinlayer-chromatographic and colorimetric techniques. In both control and cholesterol-fed animals, the gall bladder bile acids constituted about one third of the total bile solids. The main component of the bile acids of both groups of animals was chenodexycholic acid, which was predominantly conjugated with glycine. No cholic acid was present although this is the main bile acid in most mammals. The major difference in bile composition between control and cholesterol-fed animals was the conjugation pattern of chenodeoxycholic acid. The ratio of glycochenodeoxycholic to taurochenode-oxycholic acid was high, 6.4, for control animals, and decreased to 2.4 for the cholesterol-fed, anemic animals. Impaired liver function, limited availability of glycine, and greater efficiency of taurocholanic acids for the disposal of excess cholesterol may be involved in the mechanism for this phenomenon.

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Authors and Affiliations

  1. Department of Nutritional Sciences, University of California, 94720, Berkeley, California

    Jwu-Sheng Tung & Rosemarie Ostwald

Authors
  1. Jwu-Sheng Tung
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  2. Rosemarie Ostwald
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Additional information

Material in this paper has been submitted in partial satisfaction of the requirements for the degree of Master of Science in Nutition in the Graduate Division of the University of California, Berkeley.

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Tung, JS., Ostwald, R. Effect of dietary cholesterol on bile-acid composition of gall bladder bile from guinea pigs. Lipids 4, 216–223 (1969). https://doi.org/10.1007/BF02532632

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  • DOI: https://doi.org/10.1007/BF02532632

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