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Regulation of lipid metabolism in in vitro cultured minimal deviation hepatoma 7288C1

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Lipids

Abstract

Hepatoma tissue culture (HTC) cells (derived from minimal deviation hepatoma 7288C) were cultivated in a complete medium containing either glucose, fructose or glycerol as the primary carbon source. Growth was rapid on glucose and very low on fructose containing media. Glycerol did not support any growth. Glucose-14C (U) and fructose-14C (U) are incorporated into the total lipid fraction of HTC cells. However the level of conversion of glucose into lipid is much greater than fructose. Tritiated water is rapidly incorporated into the saponifiable and nonsaponifiable lipid fractions of growing HTC cells. The level of incorporation is greater than that observed with glucose-14C (U) and the difference is constant over the experimental period studied. Lipoprotein poor serum (LPPS) isolated from a calf-fetal calf serum mixture (1∶1) supported growth at a similar rate as the unfractionated serum combination (DS). However the total lipid and total cholesterol content of the fractionated serum was one sixth and one fiftieth the level found in the whole serum mixture, respectively. The level of incorporation of glucose-14C (U), acetate-14C (U) and tritiated water into the nonsaponifiable fraction of HTC cells grown on LPPS was 3 to 8-fold greater than that for DS. However mevalonate-2-14C incorporation was stimulated only 1.3 to 1.7-fold. In general there was a much smaller response in the level of incorporation of radioactive metabolites into the saponifiable lipids. From these studies and additional data, it was tentatively concluded that HTC cells can respond to nutritional pertubations caused by changes in the exogenous lipid content. It was not determined if the apparent responsiveness in the lipids of the nonsaponifiable fraction is due to “feedback control” or some other regulatory mechanism.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California San Francisco, 94122, San Francisco, California

    John A. Watson

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  1. John A. Watson
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Watson, J.A. Regulation of lipid metabolism in in vitro cultured minimal deviation hepatoma 7288C1 . Lipids 7, 146–155 (1972). https://doi.org/10.1007/BF02532603

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  • DOI: https://doi.org/10.1007/BF02532603

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