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A micromethod for the stereospecific determination of triglyceride structure

  • Published:
Lipids

Abstract

Triglyceride lipase and diglyceride kinase can be used in a sensitive stereospecific analysis of the separate fatty acid compositions at the 1, 2 and 3 positions of a triglyceride.

Diglyceride kinase fromEscherichia coli selectively catalyzes the phosphorylation of 1,2-diglycerides but not the 2,3-diglycerides.

The composition of the 3-position in rat liver triglycerides is clearly different from that at the 1-position.

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Author information

Author notes

  1. Sister P. M. Slakey (National Science Foundation Graduate Fellow)

    Present address: Badische Anilin and Soda Fabrik, Ludwigshofen, Germany

Authors and Affiliations

  1. The University of Michigan, Ann Arbor, Michigan

    William E. M. Lands, Ronald A. Pieringer & Sister P. M. Slakey (National Science Foundation Graduate Fellow)

  2. School of Medicine, Temple University, Philadelphia, Pennsylvania

    William E. M. Lands, Ronald A. Pieringer & Sister P. M. Slakey (National Science Foundation Graduate Fellow)

  3. Badische Anilin and Soda Fabrik, Ludwigshofen, Germany

    Albrecht Zschocke

Authors
  1. William E. M. Lands
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  2. Ronald A. Pieringer
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  3. Sister P. M. Slakey
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  4. Albrecht Zschocke
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Additional information

A preliminary report of this work has been presented (1).

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Lands, W.E.M., Pieringer, R.A., Slakey, S.P.M. et al. A micromethod for the stereospecific determination of triglyceride structure. Lipids 1, 444–448 (1966). https://doi.org/10.1007/BF02532550

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  • DOI: https://doi.org/10.1007/BF02532550

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