Abstract
The methylating activity of (methyl-14C)-S-adenosylmethionine by microsomes from different stages of development of the insectCeratitis capitata was studied in a series of in vitro experiments. Larval and pharate adult microsomal preparations were used in the in vitro conditions, and the utilization of the methyl group of the S-adenosylmethionine for the synthesis of phospholipids was evaluated. Incorporation of radio-activity in lipids by pharate adult microsomes was significantly higher than that by larval preparations. In both cases, phosphatidyl ethanolamine showed the highest levels of radioactivity incorporation. Free bases from total lipid hydrolysates were resolved by paper chromatography, and the labeling was investigated by radioactivity scanning of paper chromatograms. Larval and pharate adult preparations showed clearly the presence of dimethylethanolamine and choline. The presence in the incubation media of phosphatidyldimethylethanolamine and deoxycholate enhanced the radioactivity incorporation into choline, whereas, the first stages of methylation were inhibited. These findings confirmed previous results using insect homogenates.
Similar content being viewed by others
References
Jimenez, C., A.M. Municio, and A. Suarez, Lipids 10:82 (1975).
Municio, A.M., J.M. Odriozola, and A. Piñeiro, Comp. Biochem. Physiol. 37:387 (1970).
Bourre, J.M., S.A. Pollet, O.L. Daudu, and N.A. Baumann, C. R. Acad. Sci. Ser. D., 273:1534 (1971).
Bligh, E.G., and W.J. Dyer, Can. J. Biochem. Physiol. 39:911 (1959).
Cooksay, K.E., and D.M. Greenberg, Biochem. Biophys. Res. Comm. 6:256 (1961).
Author information
Authors and Affiliations
About this article
Cite this article
Jimenez, C., Municio, A.M. & Suarez, A. Methylating activity of (Methyl-14C)-S-adenosylmethionine by microsomes of the insectCeratitis capitata . Lipids 10, 532–534 (1975). https://doi.org/10.1007/BF02532355
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02532355