Abstract
Procedures for the large scale isolation of pure triglycerides and fractions rich in nontriglyceride components from Span rapeseed oil are described. Fractionation ofBrassica campestris var. Span rapeseed oil by molecular distillation yielded 4 triglyceride fractions, all of which contained traces of sterol esters. An additional triglyceride fraction rich in free and esterified sterols and other volatile components was obtained from the oil. Separation by adsorption chromatography of Span rapeseed oil yielded three fractions: A) a pure triglyceride fraction; B) a triglyceride fraction rich in sterol esters; and C) another fraction containing free sterols and other polar components.
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References
Rocquelin, G., and R. Cluzan, Ann. Biol. Anim. Biochim. Biophys. 8:395 (1968).
Rocquelin, G., B. Martin, and R. Cluzan, Proceedings of the International Conference on the Science, Technology and Marketing of Rapeseed and Rapeseed Products, Sept. 20–23, St. Adele, Quebed, Canada, 1970 pp. 405–422.
Kramer, J.K.G., S. Mahadevan, J.R. Hunt, F.D. Sauer, A.H. Corner, and K.M. Charlton, J. Nutr. 103:1696 (1973).
Rocquelin, G., J.-P. Sergiel, P.O. Astorg, and R. Cluzan, Ann. Biol. Anim. Biochim. Biophys. 13:587 (1973).
Rocquelin, G., R. Cluzan, N. Vodovar, and R. Levillain, Cah. Nutr. Diét 8:103 (1973).
Abdellatif, A.M.M., and R.O. Vles, Nutr. Metabol. 15:219 (1973).
Kramer, J.K.G., H.W. Hulan, S. Mahadevan, F.D. Sauer, and A.H. Corner, Lipids 10:511 (1975).
Stoffel, W., F. Chu., and E.H. Ahrens, Anal. Chem. 31:307 (1959).
Christie, W.W., “Lipid Analysis,” Pergamon Press, Oxford, England, 1973, p. 96.
Beare-Rogers, J.L., E.A. Nera, and B.M. Craig, Lipids 7:46 (1972).
Appelqvist, L.-0A. and R. Ohlson, Editors, “Rapeseed,” Elsevier Publishing Co., Amsterdam, The Netherlands, 1972, pp. 123–197.
Kukis, A., and J.M.R. Beveridge, J. Lipid Res. 1:311 (1960).
Privett, O.S., M.L. Blank, and W.O. Lundberg, JAOCS 38:312 (1961).
Markley, K.S. “Fatty Acids, Their Chemistry, Properties, Production, and Uses,” Part 3, Interscience Publishers, New York, N.Y., 1964, pp. 2065–2071.
Holló, J., E. Kurucz, and A. Boródi, “The Applications of Molecular Distillation,” translated by A. Páll and D.A. Durham, Akadémiai Kiadó, Budapest, Hungary, 1971, pp. 61–75.
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Contribution no. 559 Animal Research Institute.
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Kramer, J.K.G., Hulan, H.W., Mahadevan, S. et al. Brassica campestris var. Span: I. Fractionation of Rapeseed oil by molecular distillation and adsorption chromatography. Lipids 10, 505–510 (1975). https://doi.org/10.1007/BF02532350
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DOI: https://doi.org/10.1007/BF02532350