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Visualization of protein kinase activities in single cells by antibodies against phosphorylated vimentin and GFAP

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Abstract

Vimentin and glial fibrillary acidic protein (GFAP) are intermediate filament proteins expressed in the cytoplasm of various types of cells. The head domains of these proteins are phosphoralated by various protein kinases. Site- and phosphorylation-specific antibodies which recognize a phosphorylated serine/threonine residue in the head domains and its flanking sequence provide a useful tool to monitor and visualize protein kinase activities in single cells.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464, Aichi, Japan

    Naoyuki Inagaki, Jin Tanaka & Masaki Inagaki

  2. Laboratory of Immunology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464, Aichi, Japan

    Kunio Tsujimura

  3. Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, 173, Tokyo, Japan

    Masayuki Sekimata & Yoshimasa Kamei

Authors
  1. Naoyuki Inagaki
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  2. Kunio Tsujimura
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  3. Jin Tanaka
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  4. Masayuki Sekimata
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  5. Yoshimasa Kamei
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  6. Masaki Inagaki
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Dedicated to Prof. Hans Theoenen who supported N. I.

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Inagaki, N., Tsujimura, K., Tanaka, J. et al. Visualization of protein kinase activities in single cells by antibodies against phosphorylated vimentin and GFAP. Neurochem Res 21, 795–800 (1996). https://doi.org/10.1007/BF02532302

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  • DOI: https://doi.org/10.1007/BF02532302

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