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Incorporation and metabolism of fatty acids by cultured dissociated cells from rat cerebrum

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Lipids

Abstract

Dissociated brain cells in culture incorporate a variety of saturated and unsaturated fatty acids into their cellular lipids. Of the various fatty acids studied, uptake of radioactivity was greatest for stearic acid and decreased progressively with decreasing chain length. Incorporation of radioactivity from linoliec and linolenic acids was more extensive than from oleic acid. Cellular phospholipids and triacylglycerols were labeled preferentially from all fatty acid precursors, with the relative amount of label in phospholipids being greatest when cells were incubated with linolenic acid. Fatty acids underwent desaturation and chain elongation. Changes in the labeling pattern of phospholipid fatty acids in the course of incubation demonstrated precursor-product relationships for laurate (12∶0), myristate (14∶0), palmitate (16∶0), and stearate (18∶0) and for linolenate (18∶3), eicosapentaenoate (20∶5), docosapentaenoate (22∶5), and docosahexaenoate (22∶6). The appearance of label in 22∶5 and 22∶6 paralleled the entrance of label into the ethanolamine phosphoglyceride fraction. Conversion of linoleate (18∶2 ω6) to arachidonate (20∶4 ω6) could be demonstrated but did not proceed via 18∶3 ω6.

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Authors and Affiliations

  1. Division of Pediatric Neurology, University of California at Los Angeles and Brentwood Veterans' Hospital, Los Angeles, California

    Ephraim Yavin & John H. Menkes

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  1. Ephraim Yavin
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  2. John H. Menkes
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Yavin, E., Menkes, J.H. Incorporation and metabolism of fatty acids by cultured dissociated cells from rat cerebrum. Lipids 9, 248–253 (1974). https://doi.org/10.1007/BF02532201

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  • DOI: https://doi.org/10.1007/BF02532201

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