, Volume 10, Issue 2, pp 113–115 | Cite as

Separation of polyunsaturated fatty acids by argentation thin layer chromatography

  • Peter A. Dudley
  • Robert E. Anderson
Short Communications


A single step, silver nitrate thin layer chromatographic procedure which separates a methyl ester mixture containing 0–6 double bonds is described. Purity of each ester recovered from the silver nitrate plate was 98–99%. Recovery of the esters ranged from 100% for saturates to 77% for pentaenes.


Electron Spin Resonance Fatty Acid Methyl Ester Thin Layer Plate Pentaenes Arachidate 
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  1. 1.
    Blank, M.L., B. Verdino, and O.S. Privett, JAOCS 42:87 (1965).PubMedGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1975

Authors and Affiliations

  • Peter A. Dudley
    • 1
  • Robert E. Anderson
    • 1
  1. 1.Department of OphthalmologyBaylor College of MedicineHouston

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