, Volume 5, Issue 4, pp 385–391 | Cite as

Correlation between post-heparin lipase and phospholipase activities in human plasma

  • William C. Vogel
  • Edwin L. Bierman


The hypothesis that a single lipolytic enzyme in post-heparin plasma effects the hydrolysis of both triglyceride and phospholipid was tested. After intravenous heparin, activity in plasma with the two substrate classes appeared and disappeared in parallel. The activities were not separable by the fractionation methods of zone electrophoresis, gel filtration, anion-exchange, ultracentrifugation, or by combinations of these techniques. The degree of purification of the two activities with the use ofn-butanol was similar. Lipolytic activity appeared to be associated with a large high density molecular aggregate. However, the concept of a single post-heparin enzyme does not explain all the observations since the ratio of activity with triglyceride substrate to activity with phospholipid substrate decreased markedly in some subjects after increased amounts of intravenous heparin.


Lipolytic Activity Phosphatidyl Choline Phosphatidyl Ethanolamine Intravenous Heparin Phospholipase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Vogel, W. C., and L. Zieve, J. Lipid Res. 5:177–183 (1964).PubMedGoogle Scholar
  2. 2.
    Vogel, W. C., and E. L. Bierman, Ibid. 8:46–53 (1967).PubMedGoogle Scholar
  3. 3.
    Vogel, W. C., W. G. Ryan, J. L. Koppel and J. H. Olwin, Ibid. 6:335–340 (1965).PubMedGoogle Scholar
  4. 4.
    Infante, R., J. Polonovski and O. Donon, Biochim. Biophys. Acta 144:490–492 (1967).PubMedGoogle Scholar
  5. 5.
    Infante, R., K. Koumanov and J. Polonovski, Ibid. 164:436–438 (1968).PubMedGoogle Scholar
  6. 6.
    Vogel, W. C., and E. L. Bierman, Proc. Soc. Exptl. Biol. Med. 127:77–80 (1968).Google Scholar
  7. 7.
    van den Bosch, H., and L. L. M. van Deenen, Biochim. Biophys. Acta 84:234–236 (1964).Google Scholar
  8. 8.
    van den Bosch, H., N. M. Postema, G. H. DeHaas and L. L. M. van Deenen, Ibid. 98:657–659 (1965).PubMedGoogle Scholar
  9. 9.
    DeHaas, G. H., L. Sarda and J. Roger, Ibid. 106:638–640 (1965).Google Scholar
  10. 10.
    Frederickson, D. S., K. Ono and L. L. Davis, J. Lipid Res. 4:24–33 (1963).Google Scholar
  11. 11.
    Rizack, M. A., Proc. Soc. Exptl. Biol. Med. 104:111–113 (1960).Google Scholar
  12. 12.
    Singleton, W. S., M. S. Gray, M. L. Brown and J. L. White, JAOCS 42:53–56 (1965).PubMedGoogle Scholar
  13. 13.
    Dole, V. P., and H. Meinertz, J. Biol. Chem. 235:2595–2599 (1960).PubMedGoogle Scholar
  14. 14.
    Bierman, E. L., E. Gordis and J. T. Hamlin, III, J. Clin. Invest. 41:2254–2260 (1962).PubMedCrossRefGoogle Scholar
  15. 15.
    Folch, J., M. Lees and G. H. Sloane-Stanley, J. Biol. Chem. 226:495–509 (1957).Google Scholar
  16. 16.
    Flodin, P., and J. Killander, Biochim. Biophys. Acta. 63:403–410 (1962).CrossRefGoogle Scholar
  17. 17.
    Gelotte, B., P. Flodin and J. Killander, Arch. Biochem. Biophys. Suppl. 1:319–326 (1962).PubMedGoogle Scholar
  18. 18.
    Franzini, C., Clin. Chim. Acta 14:576–578 (1966).PubMedCrossRefGoogle Scholar
  19. 19.
    Morton, R. K., Nature, 166:1092–1095 (1950).PubMedCrossRefGoogle Scholar
  20. 20.
    Melius, P., and W. S. Simmons, Biochim. Biophys. Acta 105:600–602 (1965).PubMedGoogle Scholar
  21. 21.
    Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall, J. Biol. Chem. 193:265–275 (1951).PubMedGoogle Scholar
  22. 22.
    Shore, B., and V. Shore, J. Atherosclerosis Res. 2:104–114 (1962).CrossRefGoogle Scholar
  23. 23.
    Nikkila, E. A., Scand. J. Clin. Lab. Invest. Suppl. 8, 5:64 (1953).Google Scholar
  24. 24.
    Shore, B., and V. Shore, Am. J. Physiol. 201:915–922 (1961).PubMedGoogle Scholar
  25. 25.
    Shore, B., and V. Shore, Nature, 193:163–164 (1962).PubMedCrossRefGoogle Scholar
  26. 26.
    Greten, H., R. I. Levy and D. S. Fredrickson, Biochim. Biophys. Acta 164:185–194 (1968).PubMedGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1969

Authors and Affiliations

  • William C. Vogel
    • 1
    • 2
  • Edwin L. Bierman
    • 1
    • 2
  1. 1.Medical ServiceVeterans Administration HospitalSeattle
  2. 2.Department of MedicineUniversity of Washington School of MedicineSeattle

Personalised recommendations