Abstract
An automated thermogravimetric system for the microdetermination of lipids is described. A study of the analytical variability experienced with this method revealed that samples can be assayed with considerable accuracy (±3%) down to 100–200 μg, while samples less than this range exhibit variations of ±7–10%. In the absence of other more sensitive methods, even this experimental error is acceptable. In addition, multiple applications of very dilute samples to achieve a lipid mass with an acceptable coefficient of variation is possible. No significant variations were observed with samples assayed on the same day and on different days, indicating a high degree of control of nonoperator errors. A careful application of the method to different lipid classes revealed no significant weighing losses under the experimental conditions of this study, except for cholesterol; however, application of the method to mixtures of lipids containing significant amounts of cholesterol did not reveal abnormal errors.
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Leerkamp, J., Siakotos, A.N. Mass determination of lipids with automated thermogravimetric analytical procedure. Lipids 9, 415–418 (1974). https://doi.org/10.1007/BF02532059
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DOI: https://doi.org/10.1007/BF02532059