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Separation of cholesterol-desmosterol acetates by thin-layer and column chromatography on silica gel G-silver nitrate

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Lipids

Abstract

A chromatographic method, using columns packed with Celite-Silica Gel G impregnated with silver nitrate, has been developed for the separation of cholesterol from desmosterol (24-dehydrocholesterol).

The system employed allows a complete separation of the two sterols as acetates, as determined by gas-liquid chromatography and thin-layer chromatography.

The recovery of pure cholesterol and desmosterol is quantitative.

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Authors and Affiliations

  1. Institute of Pharmacology, University of Milan, Milan, Italy

    Giovanni Galli & Enrica Grossi Paoletti

Authors
  1. Giovanni Galli
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  2. Enrica Grossi Paoletti
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Galli, G., Paoletti, E.G. Separation of cholesterol-desmosterol acetates by thin-layer and column chromatography on silica gel G-silver nitrate. Lipids 2, 72–75 (1967). https://doi.org/10.1007/BF02532004

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  • DOI: https://doi.org/10.1007/BF02532004

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