, Volume 2, Issue 1, pp 60–63 | Cite as

Role of ATP in the inhibition of lipogenesis in fasted animals

  • S. Rous
  • L. Luthi
  • P. Favarger


This study was undertaken to obtain information both in vivo and in vitro on the role of adenosine triphosphate (ATP) in the inhibition of fatty acid synthesis in fasted mice.

Fasted mice were injected intravenously with glucose or fructose or intraperitoneally with insulin to increase the supply of endogenous ATP. They then received acetate 1−14C, glucose 6−3H or fructose U−14C intravenously and were killed at various intervals. The controls received the labeled tracers only. The radioactivities of liver and carcass fatty acids were determined.

The action of ATP on homogenized livers from fasted mice was also determined.

The stimulation of lipogenesis was obtained under all these conditions, but only in animals fasted for 4 hr. Insulin was active only on the extrahepatic tissues. Fructose, as well as glucose, restored hepatic lipogenesis. In vitro, ATP restored lipogenesis by homogenized livers of mice fasted for 4 hr, but it inhibited the fatty acid synthesis by homogenized livers from unfasted mice.

The significance of the results is discussed.


Fructose Fatty Acid Synthesis Adenosine Triphosphate Hepatic Glycogen POPOP 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Catravas, G. N., and H. S. Anker, J. Biol. Chem.232, 669–680 (1958).PubMedGoogle Scholar
  2. 2.
    Bortz, W. M., and F. Lynen, Federation Proc.23, Part I, 166 (1964).Google Scholar
  3. 3.
    Langdon, R. G., in “Lipid Metabolism,” Ed. K. Bloch, J. Wiley, New York, 1960, p. 238–290.Google Scholar
  4. 4.
    Bortz, W. M., and F. Lynen, Biochem. Z.337, 505–509 (1963).PubMedGoogle Scholar
  5. 5.
    Lorch, E., S. Abraham and I. L. Chaikoff, Biochim. Biophys. Acta70, 627–641 (1963).PubMedCrossRefGoogle Scholar
  6. 6.
    Korchak, H. W., and E. J. Masoro, Biochim. Biophys. Acta84, 750–753 (1964).PubMedGoogle Scholar
  7. 7.
    Masoro, E. J., and J. M. Felts, J. Biol. Chem.234, 198–200 (1959).PubMedGoogle Scholar
  8. 8.
    Tepperman, H. M., and J. Tepperman, Diabetes7, 478–485 (1958).PubMedGoogle Scholar
  9. 9.
    Gibson, D. M., and D. D. Hubbard, Biochem. Biophys. Res. Commun.3, 531–535 (1960).PubMedCrossRefGoogle Scholar
  10. 10.
    Numa, S., M. Matsuhashi and F. Lynen, Biochem. Z.334, 203–217 (1961).PubMedGoogle Scholar
  11. 11.
    Korchak, H. M., and E. J. Masoro, Biochim. Biophys. Acta58, 354–356 (1962).PubMedCrossRefGoogle Scholar
  12. 12.
    Fritz, I. B., Physiol. Rev.41, 52–129 (1961).PubMedGoogle Scholar
  13. 13.
    Knoche, I., and F. Hartmann, Biochem. Z.334, 269–278 (1961).PubMedGoogle Scholar
  14. 14.
    Pressman, B. C., and H. A. Lardy, J. Biol. Chem.197, 547–556 (1952).PubMedGoogle Scholar
  15. 15.
    Hülsmann, W. C., W. B. Elliot and E. C. Slater, Biochim. Biophys. Acta39, 267–276 (1960).PubMedCrossRefGoogle Scholar
  16. 16.
    Hommes, F. A., and J. A. C. M. Van der Beek, Biochem. Biophys. Res. Commun.13, 340–347 (1963).CrossRefGoogle Scholar
  17. 17.
    Azzone, G. F., and L. Ernster, J. Biol. Chem.236, 1501–1509 (1961).PubMedGoogle Scholar
  18. 18.
    Masoro, E. J., Ann. N. Y. Acad. Sci.131, 199–206 (1965).PubMedGoogle Scholar
  19. 19.
    Masoro, E. J., Am. J. Physiol.199, 449–452 (1960).PubMedGoogle Scholar
  20. 20.
    Holt von, C., and H. Bühring, Biochem. Z.335, 582–594 (1962).Google Scholar
  21. 21.
    Hendler, R. W., Analyt. Biochem.7, 110–120 (1964).PubMedCrossRefGoogle Scholar
  22. 22.
    Rafaelsen, O. J., V. Lauris and A. E. Renold, Diabetes14, 19–26 (1965).PubMedGoogle Scholar
  23. 23.
    Rous, S., and P. Favarger, VIIIèmes Journées biochimiques latines, Lisbonne 1965, “Rapports,” p. 34–81 (1965).Google Scholar
  24. 24.
    Leuthardt, F., and T. Stuhlfau, Med. Grundlagenforsch.3, 415–420 (1960).Google Scholar
  25. 25.
    Buchs, M., and P. Favarger, Helv. Physiol, Pharmacol. Acta23, 220–229 (1965).Google Scholar
  26. 26.
    Rous, S., L. Lüthi and P. Favarger, Med. Pharmacol. Exp.13, 199–205 (1965).Google Scholar
  27. 27.
    Kaziro, Y., L. F. Hass, P. D. Boyer and S. Ochoa, J. Biol. Chem.237, 1460–1468 (1962).PubMedGoogle Scholar
  28. 28.
    Wakil, S. J., J. K. Goldman, I. P. Williamson and R. E. Toomey, Proc. Nat. Acad. Sci., USA,55, 880–887 (1966).CrossRefGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1966

Authors and Affiliations

  • S. Rous
    • 1
  • L. Luthi
    • 1
  • P. Favarger
    • 1
  1. 1.Institute of Medical BiochemistryUniversity of GenevaGenevaSwitzerland

Personalised recommendations