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Quantitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids

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Lipids

Abstracts

Quantitative elution of acidic and neutral glycolipids of brain and spinach leaves from silicic acid columns with acetone was demonstrated. Cerebrosides and sulfatides of brain and sulfolipid and glycosyl diglycerides of spinach leaves were eluted quantitatively with acetone while prospholipids remained on the column. The observations provide the basis for an analytical procedure employing column and quantitative thin-layer chromatography (TLC). Sephadex column chromatography is utilized for separation of lipids from nonlipids; silicic acid column chromatography for separation into neutral lipid, glycolipid and phospholipid fractions; and quantitative TLC for analysis of lipid classes of each column fraction.

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Author information

Author notes

  1. Gerald Simon

    Present address: Department of Biochemistry, Presbyterian-St. Luke's Hospital, Chicago, Illinois

Authors and Affiliations

  1. Department of Biochemistry, City of Hope Medical Center, Duarte, California

    George Rouser, Gene Kritchevsky & Gerald Simon

  2. Bio-Medical Research Division, Lawrence Radiation Laboratory, University of California, Livermore, California

    Gary J. Nelson

Authors
  1. George Rouser
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  2. Gene Kritchevsky
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  3. Gerald Simon
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  4. Gary J. Nelson
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Rouser, G., Kritchevsky, G., Simon, G. et al. Quantitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids. Lipids 2, 37–40 (1967). https://doi.org/10.1007/BF02531998

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  • DOI: https://doi.org/10.1007/BF02531998

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