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Mechanism of lipoxidase reaction. II. Origin of the oxygen incorporated into linoleate hydroperoxide

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Lipids

Abstract

Two different series of experiments were performed to establish the origin of the oxygen molecule incorporated into hydroperoxide during the incubation of lipoxidase with linoleic acid. These showed, as previously assumed but never demonstrated, that the oxygen introduced into the hydroperoxide molecules comes from the gaseous phase and not from the aqueous phase. Furthermore, soybean lipoxidase does not catalyze the exchange between gaseous oxygen and water oxygen. Possibly, lipoxidase may be involved in the biosynthesis of hydroxytrans,cis conjugated octadecadienoates present in various seeds.

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Author information

Authors and Affiliations

  1. Northern Regional Research Laboratory, Peoria, Illinois

    Ami Dolev, W. K. Rohwedder, T. L. Mounts & H. J. Dutton

Authors
  1. Ami Dolev
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  2. W. K. Rohwedder
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  3. T. L. Mounts
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  4. H. J. Dutton
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Additional information

This work was conducted under a Postdoctoral Resident Research Associateship established at the Northern Laboratory by ARS, USDA, in association with the National Academy of Sciences-National Research Council.

No. Utiliz. Res. Dev. Div., ARS, USDA.

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Dolev, A., Rohwedder, W.K., Mounts, T.L. et al. Mechanism of lipoxidase reaction. II. Origin of the oxygen incorporated into linoleate hydroperoxide. Lipids 2, 33–36 (1967). https://doi.org/10.1007/BF02531997

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  • DOI: https://doi.org/10.1007/BF02531997

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