, Volume 8, Issue 5, pp 271–276 | Cite as

Dimorphotheca sinuata lipoxygenase: Formation of 13-L-hydroperoxy-cis-9,trans-11-octadecadienoic acid from linoleic acid

  • H. W. Gardner
  • D. D. Christianson
  • R. Kleiman


Lipoxygenase (EC from the seed ofDimorphotheca sinuata oxidized linoleic acid to predominantly 13-L-hydroperoxy-cis-9,trans-11-octadecadienoic acid. When the reaction proceeded at pH 6.9, the 13-hydroperoxide was the only isomer detected; but at pH 5.1, the 13-isomer was 92% of the total, the remaining 8% being the 9-hydroperoxide. At both pH's small amounts of hydroxyoctadecadienoic acid accumulated during the reaction. This acid from the pH 6.9 reaction was analyzed as 13-hydroxy-cis,trans-octadecadienoic. The postulate advanced by many workers that dimorphecolic acid, 9-D-hydroxy-trans-10,trans-12-octadecadienoic acid, is biosynthesized via a lipoxygenase product was not proved. Although the product specificity ofD. sinuata lipoxygenase is like that of lipoxygenase type 1 from soybeans, its inactivity at pH 9 demonstrated that it is a novel enzyme.


Linoleic Acid Lipoxygenase Activity Hydroxystearic Acid Lipoxygenase Product Linoleic Acid Hydroperoxide 
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Copyright information

© American Oil Chemists’ Society 1973

Authors and Affiliations

  • H. W. Gardner
    • 1
  • D. D. Christianson
    • 1
  • R. Kleiman
    • 1
  1. 1.Northern Regional Research LaboratoryPeoria

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