Skip to main content
Log in

Fungi pathogenic to insects: III. Neutral and polar lipids ofEntomophthora coronata

  • Published:
Lipids

Abstract

The neutral and polar lipid composition ofEntomophthora coronata was determined qualitatively. The fungus was grown on a chemically nondefined medium (Sabouraud dextrose yeast extract) and a chemically defined medium for a period up to 26 days. The lipids were characterized by thin-layer, column, gas chromatography, and selective sprays,32P-labeling, and mass spectrometry. The neutral lipids consist of monoglycerides, diglycerides, cholesterol, free fatty acids, triglycerides, and cholesteryl esters. The polar lipids consist of phospholipids (phosphatidyl ethanolamine, phosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidyl choline, and spingomyelin), a number of glycolipids including cerebrosides, and many unrecognizable lipids, most of which are present in trace amounts. The cerebrosides and spingomyelin are present in significant amounts, and their concentration increased with age of the culture. The major fatty acids (>10%) of the total, neutral, and polar lipids of the mycelia are 14∶0, 16∶0, 18∶1, 18∶3(γ), and 24∶1. The polar lipids of total culture (unsaturation index 0.88) and of the conidia (unsaturation index 1.48) are considerably more unsaturated than the corresponding neutral lipids (unsaturated index 0.50 and 0.49). The mycelial polar lipids, compared to the neutral lipids, possess less 14∶0 and 18∶1 but contain a greater percentage of 16∶0, 18∶2, 18∶3(γ), 24∶0, and 24∶1. The major fatty acid of the conidia (>10%) are 13∶0, 14∶0, 18∶1, 18∶2, 18∶3(γ), and 20∶4. Their polar lipids have a higher proportion of 18∶2, 18∶3(γ), and 20∶4. The cerebrosides possess 24.1 in high relative proportion (30.1%).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tyrrell, D., Can. J. Microbiol. 13:755 (1967).

    Article  PubMed  CAS  Google Scholar 

  2. Tyrrell, D., Botanical Rev. 35:305 (1969).

    Article  CAS  Google Scholar 

  3. Mumma, R.O., and T.E. Bruszewski, Lipids 5:915 (1970).

    Article  PubMed  CAS  Google Scholar 

  4. Bras, G., C.C. Gordon, C.W. Emmons, K.M. Prendegast and M. Sugar, Amer. J. Trop. Med. Hyg. 14:141 (1965).

    CAS  Google Scholar 

  5. Mumma, R.O., and T.E. Bruszewski, Paper presented in part at the AOCS Meeting, Atlantic City, October 1971.

  6. Freman, C.P., and D. West, J. Lipid Res. 7:324 (1966).

    Google Scholar 

  7. Parsons, J.G., and S. Patton, Ibid. 8:696 (1967).

    PubMed  CAS  Google Scholar 

  8. Dawson, R.M.C., Biochem. J., 75:45 (1960).

    PubMed  CAS  Google Scholar 

  9. Mauro, B., and A.A. Benson, J. Biol. Chem. 234:254 (1959).

    Google Scholar 

  10. Rouser, G., G. Kritchevsky, and A. Yamamoto, in “Lipid Chromatographic Analysis,” Edited by G.V. Marinetti, Marcel Dekker, New York, N.Y., 1967, pp. 116–120.

    Google Scholar 

  11. Siakotos, A.M., and G. Rouser, JAOCS 42:913 (1965).

    PubMed  CAS  Google Scholar 

  12. Vance, D.E., and C.C. Sweeley, J. Lipid Res. 8:621 (1967).

    PubMed  CAS  Google Scholar 

  13. Folch, J., M. Lees, and G.H. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).

    PubMed  CAS  Google Scholar 

  14. Vaskovsky, V.E., and E.Y. Kostetsky, J. Lipid Res. 9:396 (1968).

    PubMed  CAS  Google Scholar 

  15. Krebs, K.G., D. Heusser and H. Wimmer. in “Thin-layer Chromatography,” Edited by E. Sthal, Academic Press, New York, N.Y., 1969, pp. 485–502.

    Google Scholar 

  16. Shaw, N., Biochim. Biophys. Acta 164:435 (1968).

    PubMed  CAS  Google Scholar 

  17. Weiss, B., and R.L. Stiller, Biochem. 11:4552 (1972).

    Article  CAS  Google Scholar 

  18. Weiss, B., and R.L. Stiller, Lipids 8:25 (1973).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

About this article

Cite this article

Mumma, R.O., Bruszewski, T.E. Fungi pathogenic to insects: III. Neutral and polar lipids ofEntomophthora coronata . Lipids 8, 745–752 (1973). https://doi.org/10.1007/BF02531843

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02531843

Keywords

Navigation