Lipids

, Volume 8, Issue 12, pp 745–752 | Cite as

Fungi pathogenic to insects: III. Neutral and polar lipids ofEntomophthora coronata

  • R. O. Mumma
  • T. E. Bruszewski
Article

Abstract

The neutral and polar lipid composition ofEntomophthora coronata was determined qualitatively. The fungus was grown on a chemically nondefined medium (Sabouraud dextrose yeast extract) and a chemically defined medium for a period up to 26 days. The lipids were characterized by thin-layer, column, gas chromatography, and selective sprays,32P-labeling, and mass spectrometry. The neutral lipids consist of monoglycerides, diglycerides, cholesterol, free fatty acids, triglycerides, and cholesteryl esters. The polar lipids consist of phospholipids (phosphatidyl ethanolamine, phosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidyl choline, and spingomyelin), a number of glycolipids including cerebrosides, and many unrecognizable lipids, most of which are present in trace amounts. The cerebrosides and spingomyelin are present in significant amounts, and their concentration increased with age of the culture. The major fatty acids (>10%) of the total, neutral, and polar lipids of the mycelia are 14∶0, 16∶0, 18∶1, 18∶3(γ), and 24∶1. The polar lipids of total culture (unsaturation index 0.88) and of the conidia (unsaturation index 1.48) are considerably more unsaturated than the corresponding neutral lipids (unsaturated index 0.50 and 0.49). The mycelial polar lipids, compared to the neutral lipids, possess less 14∶0 and 18∶1 but contain a greater percentage of 16∶0, 18∶2, 18∶3(γ), 24∶0, and 24∶1. The major fatty acid of the conidia (>10%) are 13∶0, 14∶0, 18∶1, 18∶2, 18∶3(γ), and 20∶4. Their polar lipids have a higher proportion of 18∶2, 18∶3(γ), and 20∶4. The cerebrosides possess 24.1 in high relative proportion (30.1%).

Keywords

Polar Lipid Neutral Lipid Major Fatty Acid Ethanolamine Phosphatidyl Silicic Acid Column 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Tyrrell, D., Can. J. Microbiol. 13:755 (1967).PubMedCrossRefGoogle Scholar
  2. 2.
    Tyrrell, D., Botanical Rev. 35:305 (1969).CrossRefGoogle Scholar
  3. 3.
    Mumma, R.O., and T.E. Bruszewski, Lipids 5:915 (1970).PubMedCrossRefGoogle Scholar
  4. 4.
    Bras, G., C.C. Gordon, C.W. Emmons, K.M. Prendegast and M. Sugar, Amer. J. Trop. Med. Hyg. 14:141 (1965).Google Scholar
  5. 5.
    Mumma, R.O., and T.E. Bruszewski, Paper presented in part at the AOCS Meeting, Atlantic City, October 1971.Google Scholar
  6. 6.
    Freman, C.P., and D. West, J. Lipid Res. 7:324 (1966).Google Scholar
  7. 7.
    Parsons, J.G., and S. Patton, Ibid. 8:696 (1967).PubMedGoogle Scholar
  8. 8.
    Dawson, R.M.C., Biochem. J., 75:45 (1960).PubMedGoogle Scholar
  9. 9.
    Mauro, B., and A.A. Benson, J. Biol. Chem. 234:254 (1959).Google Scholar
  10. 10.
    Rouser, G., G. Kritchevsky, and A. Yamamoto, in “Lipid Chromatographic Analysis,” Edited by G.V. Marinetti, Marcel Dekker, New York, N.Y., 1967, pp. 116–120.Google Scholar
  11. 11.
    Siakotos, A.M., and G. Rouser, JAOCS 42:913 (1965).PubMedGoogle Scholar
  12. 12.
    Vance, D.E., and C.C. Sweeley, J. Lipid Res. 8:621 (1967).PubMedGoogle Scholar
  13. 13.
    Folch, J., M. Lees, and G.H. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).PubMedGoogle Scholar
  14. 14.
    Vaskovsky, V.E., and E.Y. Kostetsky, J. Lipid Res. 9:396 (1968).PubMedGoogle Scholar
  15. 15.
    Krebs, K.G., D. Heusser and H. Wimmer. in “Thin-layer Chromatography,” Edited by E. Sthal, Academic Press, New York, N.Y., 1969, pp. 485–502.Google Scholar
  16. 16.
    Shaw, N., Biochim. Biophys. Acta 164:435 (1968).PubMedGoogle Scholar
  17. 17.
    Weiss, B., and R.L. Stiller, Biochem. 11:4552 (1972).CrossRefGoogle Scholar
  18. 18.
    Weiss, B., and R.L. Stiller, Lipids 8:25 (1973).PubMedCrossRefGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1974

Authors and Affiliations

  • R. O. Mumma
    • 1
  • T. E. Bruszewski
    • 1
  1. 1.Department of Entomology and Pesticide Research Laboratory and Graduate Study CenterThe Pennsylvania State UniversityUniversity Park

Personalised recommendations