Abstract
A cell-free system, consisting of particle-free rat liver cytoplasm plus purified mitochondria, was used to study the synthesis of fatty acid from14C-U-alanine. Incorporation of label into lipid was dependent upon the presence of both cytoplasm and mitochondria. However, the metabolic state of the animal, prior to sacrifice, determined the capacity of various mitochondrial preparations to reconstitute the system. Mitochondria prepared from the livers of starved, fat-fed, or alloxan-diabetic rats failed to support fatty acid synthesis to the same extent as mitochondria prepared from control animals. Likewise, less14CO2 was produced in the system reconstituted with liver mitochondria prepared from starved rats. Possible mechanisms are discussed.
Similar content being viewed by others
References
“Metabolic Roles of Citrate,” Edited by T.W. Goodwin, Academic Press, New York, 1968, p. 41, 61, 123.
Bhaduri, A., and P.A. Srere, J. Biol. Chem. 239:1357 (1964).
Iliffe, J., and N.B. Myant, Biochem. J. 91:369 (1964).
Watson, J.A., and J.M. Lowenstein, J. Biol. Chem. 245:5993 (1970).
Johnson, D., and H. Lardy, in “Methods of Enzymology,” Vol. X, Edited by R.W. Estabrook and M.E. Pullman, Academic Press, New York, 1967, p. 317.
Wolley, J.G., and W.H. Sebrell, J. Nutrition 39:191 (1945).
Gornall, A.G., C.H. Bardawill and M.M. David, J. Biol. Chem. 177:751 (1949).
Lowry, O.H., W.J. Rosebrough, A.L. Farr and R.J. Randall, Ibid. 193:265 (1951).
Estabrook, R.W., in “Methods in Enzymology,” Vol. X, Edited by R.W. Estabrook and M.E. Pullman, Academic Press, New York, 1967, p. 41.
Keyser, J.W., and J. Vaughn, Biochem. J. 44: XXII (1949).
Linn, T.C., F.H. Pettit, F. Hucho and L.J. Reed, Proc. Nat. Acad. Sci., U.S.A. 64:227 (1969).
Wieland, O., and E. Siess, Proc. Nat. Acad. Sci., U.S.A. 65:947 (1970).
Wieland, O., E. Siess, F.H. Schulze-Wethmar, H.G.V. Funche and B. Winto, Arch. Biochem. Biophys. 143:593 (1971).
Harris, R.A., J.M. Mahoney, J.P. Mapes and M.C. Leland, in “Studies on the Regulatory Role of the Mitochondrion in Lipogenesis,” Federation of American Societies for Experimental Biology, Atlantic City, Abstr. 2584, April 1973.
Veech, R.C., L. Raijman and H.A. Krebs, Biochem. J. 117:499 (1970).
Gibson, D.M., R.T. Lyons, D.F. Scott and Y. Muto, in “Advances in Enzyme Regulation,” Vol. 10, Edited by G. Weber, Pergamon Press, Oxford, 1972, p. 187.
Author information
Authors and Affiliations
Additional information
This research was supported in part by USPHS specialized center grant HL-14159, Heart Research Center Grant (HE-06308), and a grant from the Indiana Heart Association.
About this article
Cite this article
Harris, R.A., Leland, M.C., Mahoney, J.M. et al. Regulatory function of mitochondria in lipogenesis. Lipids 8, 711–716 (1973). https://doi.org/10.1007/BF02531837
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02531837