, Volume 8, Issue 3, pp 107–110 | Cite as

Analysis of mixtures of stigmasterol, stigmastanol and sitosterol

  • Fumiko U. Rosenstein
  • Henry W. Kircher


Hydrogenation of stigmasterol yields mixtures of the three title sterols. Stigmasterol was clearly separated from the other two by gas liquid chromatography; a standard curve was constructed relating the height of its peak to its concentration in the mixture. The total unsaturation was measured by a dibromopyridine sulfate-thiosulfate procedure. The unsaturation contributed by previously determined stigmasterol was subtracted from the total to leave that contributed by sitosterol, whose concentration in the mixture was then calculated. The amount of stigmastanol, whose presence was qualitatively determined by thin layer chromatography after bromination of the mixture on the plate, was obtained by difference. When suitable corrections were made, based on analysis of known mixtures, the concentrations of three sterols in hydrogenation mixtures could be calculated.


Sitosterol Stigmasterol Free Sterol Pivalate Stigmastanol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© American Oil Chemists’ Society 1973

Authors and Affiliations

  • Fumiko U. Rosenstein
    • 1
  • Henry W. Kircher
    • 1
  1. 1.Department of Agricultural BiochemistryThe University of ArizonaTucson

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