Skip to main content
SpringerLink
Log in

Studies on the specificity of a lipase system fromGeotrichum candidum

  • Published:
Lipids

Abstract

The lipase system fromGeotrichum candidum preferentially hydrolyzed oleic acid, regardless of position, from the four possible racemic triglycerides containing oleate and palmitate. The rate of hydrolysis of these glycerides was most rapid when the substrate contained two moles of oleate. This acid was also preferentially released from a series of triglycerides containing oleate and two moles of a saturated fatty acid. The chain length of the latter did not alter the specificity for oleate.

Equimolar quantities of oleic and linoleic acids were released when triolein and trilinolein (equimolar mixture) were hydrolyzed by this lipase. No differentiation between oleate and palmitoleate was observed when racemic glyceryl 1-palmitoleate-2,3-dioleate was the substrate. However, only 7.2 M%cis-vaccenic acid was released from glyceryl 1-cis-vaccenate-2,3-dioleate and 5.4 M% petroselinic acid from glyceryl 1-palmitoleate-2,3-dipetroselinate. It therefore appears that the enzyme may be specific forcis-9-unsaturation as well as forcis-9,cis-12-unsaturation. When specificity was assumed, the fatty acid compositions of the diglycerides obtained from digestions withG. candidum were close to theoretical.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Alford, J. A., D. A. Pierce and F. G. Suggs, J. Lipid Res.5, 390–394 (1964).

    PubMed  CAS  Google Scholar 

  2. Jensen, R. G., J. Sampugna, J. G. Quinn, D. L. Carpenter, T. A. Marks and J. A. Alford, JAOCS42, 1029–1032 (1965).

    PubMed  CAS  Google Scholar 

  3. Jensen, R. G., J. Sampugna and J. G. Quinn, Lipids1, 294–295 (1966).

    Article  CAS  PubMed  Google Scholar 

  4. Jensen, R. G., T. A. Marks, J. Sampugna, J. G. Quinn and D. L. Carpenter, Lipids1, 451–452 (1966).

    Article  CAS  PubMed  Google Scholar 

  5. Quinn, J. G., J. Sampugna and R. G. Jensen, JAOCS44, 439–442 (1967).

    CAS  Google Scholar 

  6. Alford, J. A., and J. L. Smith, JAOCS42, 1038–1040 (1965).

    PubMed  CAS  Google Scholar 

  7. Sampugna, J., R. G. Jensen, R. M. Parry, Jr., and C. F. Krewson, JAOCS41, 132–133 (1964).

    CAS  Google Scholar 

  8. Gander, G. W., R. G. Jensen and J. Sampugna, J. Dairy Sci.45, 323–328 (1962).

    Article  CAS  Google Scholar 

  9. Tinoco, J., and P. G. Miljanich, Anal. Biochem.11, 548–554 (1965).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Animal Industries, University of Connecticut, 06268, Storrs

    T. A. Marks, J. G. Quinn, J. Sampugna & R. G. Jensen

Authors
  1. T. A. Marks
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. G. Quinn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Sampugna
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. G. Jensen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Scientific contribution No. 256, Agricultural Experiment Station, University of Connecticut, Storrs.

About this article

Cite this article

Marks, T.A., Quinn, J.G., Sampugna, J. et al. Studies on the specificity of a lipase system fromGeotrichum candidum . Lipids 3, 143–146 (1968). https://doi.org/10.1007/BF02531731

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02531731

Keywords

Search

Navigation