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Extraction of prostaglandins E1, E2 and E3 from human seminal plasma

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Lipids

Abstract

Isolation of individual prostaglandins of the E group from human seminal plasma consists of three main steps: preliminary purification from other lipids; resolution of this partially purified material into various groups of prostaglandins on a column of silicic acid; and further purification and resolution of various prostaglandins of each group by reversed phase partition chromatography. The last step is time-consuming and has been replaced by a two-step thin layer chromatography: first on silica gel, which ensures purification of the PGEs, followed by argentation thin layer chromatography, which resolves them into E1, E2 and E3 in microgram quantities.

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Authors and Affiliations

  1. Department of Preventive Medicine, Hygiene and Environmental Science, Odense University, 5000, Odense

    K. C. Srivastava & J. Clausen

  2. The Neurochemical Institute, 58 Raadmandsgade, 2200, Copenhagen N, Denmark

    K. C. Srivastava & J. Clausen

Authors
  1. K. C. Srivastava
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  2. J. Clausen
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Srivastava, K.C., Clausen, J. Extraction of prostaglandins E1, E2 and E3 from human seminal plasma. Lipids 8, 431–433 (1973). https://doi.org/10.1007/BF02531721

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  • DOI: https://doi.org/10.1007/BF02531721

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