, Volume 8, Issue 7, pp 393–400 | Cite as

Preferential labeling of phosphatidylcholine during phospholipid synthesis by bovine mammary tissue

  • J. E. Kinsella


Bovine mammary cells and tissue synthesize de novo the classes of phospholipids, found in mammary tissue and milk, from various precursor molecules. Several short term experiments were carried out in vitro, using labeled precursors, i.e., 1-14C-fatty acids; 2-14C-acetate; U-14C-glycerol; 1,2-14C-choline; 1,2-14C-ethanolamine; 2-14C-serine; and Me-14C-methionine. All the phospholipid classes were labeled. The specific activity of tissue phosphatidylcholine was consistently three to six-fold greater than that of phosphatidylethanolamine. The results indicated that stepwise methylation of phosphatidylethanolamine with labeled methyl group of methionine was occurring to a minor extent, as was a negligible amount of choline exchange. Serine was incorporated into phosphatidylserine and sphingomyelin. Significant quantities of labeled phosphatidylserine were decarboxylated to phosphatidylethanolamine. Apparently phosphatidylcholine was synthesized de novo from choline via phosphorylcholine and CDP-choline. Based on the present observations and other data, it is suggested that there may be two pools of phosphatidylcholine in lactating mammary cells.


Choline Phospholipid Synthesis Label Fatty Acid Bovine Mammary Gland Bovine Mammary Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bailie, M.J., and R.K. Morton, Biochem. J. 69:35 (1958).PubMedGoogle Scholar
  2. 2.
    Stewart, P., D.L. Puppione and S. Patton, Z. Zellforsch. 123:161 (1972).PubMedGoogle Scholar
  3. 3.
    Prentice, J.H., Dairy Sci. Abstr. 31:353 (1969).Google Scholar
  4. 4.
    Swope, F.C., and J.R. Brunner, J. Dairy Sci. 53:691 (1970).Google Scholar
  5. 5.
    Patton, S., and F.M. Fowkes, J. Theor. Biol. 15:274 (1967).PubMedCrossRefGoogle Scholar
  6. 6.
    Patton, S., A. Durdan and R.D. McCarthy, J. Dairy Sci. 47:489 (1964).Google Scholar
  7. 7.
    Hayashi, S., D.R. Erickson and L.M. Smith, Biochemistry 4:2557 (1965).PubMedCrossRefGoogle Scholar
  8. 8.
    Berlin, E., S. Lakshmanan, P.G. Kliman and M.J. Pallansch, Ibid. 6:1388 (1967).PubMedCrossRefGoogle Scholar
  9. 9.
    Buckheim, W., Naturwissenschaften 12:672 (1970).CrossRefGoogle Scholar
  10. 10.
    Keenan, T.W., D.E. Olsen and H.H. Mollenhauer, J. Dairy Sci. 54:295 (1971).PubMedGoogle Scholar
  11. 11.
    Keenan, T.W., D.E. Olsen and H.H. Mollenhauer, J. Dairy Sci. 54:295 (1971).PubMedGoogle Scholar
  12. 12.
    Huang, T.C., and A. Kuksis, Lipids 1:453 (1966).Google Scholar
  13. 13.
    Nutter, L.J., and O.S. Privett, J. Dairy Sci. 50:298 (1967).PubMedGoogle Scholar
  14. 14.
    Parsons, J.P., and S. Patton, J. Lipid Res. 8:696 (1967).PubMedGoogle Scholar
  15. 15.
    Kinsella, J.E., Lipids 7:165 (1972).PubMedGoogle Scholar
  16. 16.
    Morrisson, W.R., E.L. Jack and L.M. Smith, JAOCS 42:1142 (1965).Google Scholar
  17. 17.
    Kinsella, J.E., and R.D. McCarthy, Biochim. Biophys. Acta 164:530 (1968).PubMedGoogle Scholar
  18. 18.
    Patton, S., R.O. Mumma and R.D. McCarthy, J. Dairy Sci. 49:737 (1966).Google Scholar
  19. 19.
    Patton, S., and T. Keenan, Lipids 6:58 (1971).PubMedCrossRefGoogle Scholar
  20. 20.
    Patton, S., L.F. Hood and J.S. Patton, J. Lipid Res. 10:260 (1969).PubMedGoogle Scholar
  21. 21.
    Kinsella, J.E., Biochim. Biophys. Acta 164:540 (1968).PubMedGoogle Scholar
  22. 22.
    Kinsella, J.E., Lipids 5:892 (1970).PubMedGoogle Scholar
  23. 23.
    Kinsella, J.E., J. Dairy Sci. 53:1757 (1970).PubMedGoogle Scholar
  24. 24.
    Kinsella, J.E., Ibid. 53:635 (1970).Google Scholar
  25. 25.
    Easter, D., S. Patton and R.D. McCarthy, Lipids 6:844 (1971).PubMedGoogle Scholar
  26. 26.
    Ebner, K.E., C.R. Hoover, E.C. Hageman and B.L. Larson, Exp. Cell Res. 23:373 (1961).PubMedCrossRefGoogle Scholar
  27. 27.
    Kinsella, J.E., and R.D. McCarthy, Biochim. Biophys. Acta 164:518 (1968).PubMedGoogle Scholar
  28. 28.
    Folch, J., M. Lees, G.H. Sloane-Stanley, J. Biol. Chem. 226:497 (1957).PubMedGoogle Scholar
  29. 29.
    Rouser, G., A.N. Siakotos and S. Fleischer, Lipids 1:85 (1966).PubMedGoogle Scholar
  30. 30.
    Arvidson, G.A., Eue. J. Biochem. 4:478 (1967).CrossRefGoogle Scholar
  31. 31.
    Kennedy, E.P., Fed. Proc. 20:934 (1961).PubMedGoogle Scholar
  32. 32.
    Annison, E.F., J.L. Linzell, S. Fazakerley and B. Nichols, Biochem. J. 102:637 (1967).PubMedGoogle Scholar
  33. 33.
    Dimick, P.S., R.D. McCarthy and S. Patton, Biochim. Biophys. Acta 116:159 (1966).PubMedGoogle Scholar
  34. 34.
    Kinsella, J.E., J. Dairy Sci. 54:1014 (1971).PubMedGoogle Scholar
  35. 35.
    Kinsella, J.E., Biochim. Biophys. Acta 210:28 (1970).PubMedGoogle Scholar
  36. 36.
    McCarthy, R.D., and S. Patton, Ibid. 70:102 (1963).PubMedCrossRefGoogle Scholar
  37. 37.
    Bines, J.A., and R.E. Brown, J. Dairy Sci. 51:51 (1968).Google Scholar
  38. 38.
    Wright, J.D. and C. Green, Biochem. J. 123:837 (1971).PubMedGoogle Scholar
  39. 39.
    Bremer, J., P. Figard, D. Greenberg, Biochim. Biophys Acta 43:477 (1960).CrossRefGoogle Scholar
  40. 40.
    Rytter, D., J.E. Miller and W.E. Cornatzer, Ibid. 152:418 (1968).PubMedGoogle Scholar
  41. 41.
    Artom, C., Proc. Soc. Exp. Biol. Med. 132:1025 (1969).PubMedGoogle Scholar
  42. 42.
    Tinoco, J., G. Sheehan, S. Hopkins and R.L. Lyman, Lipids 5:412 (1970).PubMedGoogle Scholar
  43. 43.
    Bjornstad, P., and J. Bremer, J. Lipid Res. 7:38 (1966).PubMedGoogle Scholar
  44. 44.
    Balint, J.A., D.A. Beeler, D.H. Treble and H.L. Spitzer, Ibid. 8:486 (1967).PubMedGoogle Scholar
  45. 45.
    Stein, O., and Y. Stein, J. Cell. Biol. 40:461 (1969).PubMedCrossRefGoogle Scholar
  46. 46.
    Gurr, M.I., D.I. Brindley and G. Hubscher, Biochim. Biophys. Acta 98:486 (1965).PubMedGoogle Scholar
  47. 47.
    Pasternak, C.A., and J.J. Bergeron Biochem. J. 119:473 (1970).PubMedGoogle Scholar
  48. 48.
    Kinsella, J.E., J. Dairy Sci. 52:11 (1969).Google Scholar
  49. 49.
    Isozaki, M., A. Yamamoto, T. Amako, Y. Sakai and H. Okita, Med. J., Osaka Univ. 12:285 (1962).Google Scholar
  50. 50.
    Collins, F.D., Nature 186:366 (1960).PubMedCrossRefGoogle Scholar
  51. 51.
    Mudd, J.B., L.M. Van Golde, L.L.M. Van Deenan, Biochim. Biophys. Acta 176:547 (1969).PubMedGoogle Scholar
  52. 52.
    Suzuki, S., M. Kobayashi and Y. Kotake, J. Vitaminol. 6:107 (1970).Google Scholar
  53. 53.
    Morin, R.J., Experientia 26:829 (1970).PubMedCrossRefGoogle Scholar
  54. 54.
    Young, D.L., G. Powell and W.O. McMillan, J. Lipid Res. 12:1 (1971).PubMedGoogle Scholar
  55. 55.
    Tata, J.R., Biochem. J. 116:617 (1970).PubMedGoogle Scholar
  56. 56.
    Oka, T., and Y.J. Topper, J. Biol. Chem. 246:7701 (1971).PubMedGoogle Scholar
  57. 57.
    Keenan, T.W., S. Patton and R. Saacke, J. Dairy Sci. 54:1349 (1971).Google Scholar
  58. 58.
    Patton, S., and L.F. Hood, in “Lactogenesis: the Initiation of Milk Secretion at Parturition,” Edited by M. Reynolds and S.J. Folley, University of Pennsylvania Press, Philadelphia, Pa., 1969, p. 12.Google Scholar
  59. 59.
    Hood, L.F., and S. Patton, J. Dairy Sci., In press 1973.Google Scholar
  60. 60.
    Keenan, T.W., and C.M. Huang, Ibid. 55:1586 (1972).PubMedGoogle Scholar
  61. 61.
    Palmquist, D.L., and R.L. Baldwin, Ibid. 54:1505 (1971).PubMedCrossRefGoogle Scholar
  62. 62.
    Gammack, D., and B.B. Gupta, Biochem. J. 103:729 (1967).Google Scholar
  63. 63.
    Jones, A.L., N. Ruderman and M.G. Herrera, J. Lipid Res. 8:429 (1967).PubMedGoogle Scholar
  64. 64.
    Gurr, M.I., D.I. Bridley and G. Hubscher, Biochim. Biophys. Acta 98:486 (1965).PubMedGoogle Scholar
  65. 65.
    Zilversmit, D.G., J. Lipid Res. 12:36 (1971).PubMedGoogle Scholar
  66. 66.
    McMurray, W.C., and R.M.C. Dawson, Biochem. J. 112:108 (1970).Google Scholar
  67. 67.
    Jungalwala, F.G., and R.M.C. Dawson, Ibid. 117:481 (1970).PubMedGoogle Scholar
  68. 68.
    Patton, S., R.D. McCarthy, P.E. Plantz and R. Lee, Nature 241:240 (1973).Google Scholar

Copyright information

© American Oil Chemists’ Society 1973

Authors and Affiliations

  • J. E. Kinsella
    • 1
  1. 1.Department of Food ScienceCornell UniversityIthaca

Personalised recommendations