Abstract
Gas liquid radiochromatography was successfully applied to the simultaneous analysis of mass and radioactivity of intact standard and natural triglycerides labeled with14C in their fatty acid or glycerol moieties. Quantitative measurements of mass and radioactivity were made with a gas chromatograph equipped with dual glass columns, stream splitters, combustion train, and a proportional gas counter. Under optimum conditions, as little as 500 cpm/peak of tristearin could be detected with a relative error of 10%. At higher counting rates the error was less than 5% and the achieved resolution of radioactivity was only slightly inferior to that of the mass. The counting efficiency of the system was better than 90%. The study showed that the detected radioactivity was proportional to the mass and not the moles of the labeled triglyceride measured in the hydrogen flame ionization detector. Practical application of the system is limited by the need for samples of relatively high specific activity (500–1000 dpm/50 μg/peak).
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Breckenridge, W.C., Kuksis, A. Gas liquid radiochromatography of intact natural triglycerides. Lipids 5, 342–352 (1970). https://doi.org/10.1007/BF02531467
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DOI: https://doi.org/10.1007/BF02531467