Abstract
A condensation reaction between serine and palmitoyl CoA by a partially-purified enzyme from rat brain is described. The product of the reaction, 3-keto-dihydrosphingosine is characterized by the conversion to several derivatives. The addition of EDTA to the incubation mixture results in inhibition of the conversion of serine to phospholipid, with the result that 3-keto-dihydro-sphingosine is the sole product.
This is a preview of subscription content, log in via an institution to check access.
References
Braun, P.E., and E.E. Snell, Proc. Nat. Acad. Sci. 58:298 (1967).
Braun, P.E., and E.E. Snell, J. Biol. Chem. 243:3775 (1968).
Brady, R.N., S.J. DiMari and E.E. Snell, J. Biol. Chem. 244:491 (1969).
Stoffel, W., D. LeKim and G. Sticht, H.S. Zeit. Physiol. Chem. 349:664 (1968).
Braun, P.E., P. Morell and N.S. Radin, J. Biol. Chem. 245:335 (1970).
Kanfer, J.N., “International Symposium on Sphingolipids”, Edited by C.C. Sweeley, in press.
Kornberg, A., and W.E. Pricer, J. Biol. Chem. 204:329 (1953).
Garver, R.C., and C.C. Sweeley, J. Amer. Chem. Soc. 88:3644 (1966).
Menderhausen, P.B., and C.C. Sweeley, Biochemistry 8:2633 (1969).
Martensson, E., and J.N. Kanfer, J. Biol. Chem. 243:497 (1968).
Sambasivarao, K., and R.H. McCluer, J. Lipid Res. 4:106 (1963).
Michalic, C., J. Chromatog. 24:228 (1966).
Rosenberg, A., and N. Stern, J. Lipid Res. 1:122 (1966).
Author information
Authors and Affiliations
About this article
Cite this article
Kanfer, J.N., Bates, S. Sphingolipid metabolism II. The biosynthesis of 3-keto-dihydrosphingosine by a partially-purified enzyme from rat brain. Lipids 5, 718–720 (1970). https://doi.org/10.1007/BF02531441
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02531441