Advertisement

Lipids

, Volume 5, Issue 9, pp 751–756 | Cite as

Fatty acid and long chain base composition of adrenal medulla gangliosides

  • Robert Ledeen
  • Kenneth Salsman
Article

Abstract

Five ganglioside fractions from bovine adrenal medulla were analyzed with respect to their fatty acid and long chain base compositions. The five fractions included two hematosides and three hexasamine-containing species, the latter having chromatographic properties comparable to the major gangliosides of brain. The fatty acid compositions of all five were similar: 22∶0 was the most abundant, but significant amounts of 16∶0, 18∶0, 24∶0 and 24∶1 were also present. No hydroxy fatty acids were detected. The principal long chain base in each fraction was 4-sphingenine (sphingosine), with lesser amounts of the C16 and C17 homologues. Minor quantities of the corresponding saturated bases were also detected. These were identified by two gas liquid chromatography methods: (a) trimethylsilyl ether derivatives, (b) aldehydes formed by periodate oxidation of the long chain bases. No 4-eicosasphingenine (C20-sphingosine), characteristic of brain gangliosides, was found in any of the fractions. The results demonstrate that gangliosides of the adrenal medulla show tissue specificity in fatty acid and long chain base composition which is independent of carbohydrate structure.

Keywords

Adrenal Medulla Hydroxy Fatty Acid Periodate Oxidation Sphinganine Brain Ganglioside 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ledeen, R., K. Salsman and M. Cabrera, Biochemistry 7:2287 (1968).PubMedCrossRefGoogle Scholar
  2. 2.
    Suzuki, K., Life Sci. 3:1227 (1964).PubMedCrossRefGoogle Scholar
  3. 3.
    Gaver, R. C., and C. C. Sweeley, JAOCS 42:294 (1965).PubMedGoogle Scholar
  4. 4.
    Carter, H. E., and R. C. Gaver, J. Lipid Res. 8:391 (1967).PubMedGoogle Scholar
  5. 5.
    Sweeley, C. C., and E. A. Moscatelli, Ibid. 1:40 (1959).Google Scholar
  6. 6.
    Karlsson, K., Acta Chem. Scand. 18:2395 (1964).CrossRefGoogle Scholar
  7. 7.
    Popovic, M., Biochim. Biophys. Acta 125:178 (1966).PubMedGoogle Scholar
  8. 8.
    Gilliland, K. M., and E. A. Moscattelli, Ibid.:221 (1969).PubMedGoogle Scholar
  9. 9.
    Rosenberg, A., and N. Stern, J. Lipid Res. 7:122 (1966).PubMedGoogle Scholar
  10. 10.
    Siddiqui, B., and R. H. McCluer, Ibid. 9:366 (1968).Google Scholar
  11. 11.
    Sambasivarao, K., and R. H. McCluer, Ibid. 5:103 (1964).Google Scholar
  12. 12.
    Ledeen, R., JAOCS 43:57 (1966).PubMedGoogle Scholar
  13. 13.
    Ledeen, R., K. Salsman and M. Cabrera, J. Lipid Res. 9:129 (1968).PubMedGoogle Scholar
  14. 14.
    Klenk, E., and L. Georgias, Z. Physiol. Chem. 348:1261 (1967).Google Scholar
  15. 15.
    Klenk, E., and G. Padberg, Ibid. 327:249 (1962).Google Scholar
  16. 16.
    Hooghwinkel, G. J. M., B. F. Borri and G. W. Bruyn, Neurology 16:934 (1966).Google Scholar
  17. 17.
    Klenk, E., and K. Heuer, Deuth. Zh. Verdau. Stoffweckselkrkh. 20:180 (1960).Google Scholar
  18. 18.
    Johnston, P. V., and B. I. Roots, J. Lipid Res. 5:477 (1964).PubMedGoogle Scholar
  19. 19.
    Svennerholm, L., Ibid. 9:570 (1968).PubMedGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1969

Authors and Affiliations

  • Robert Ledeen
    • 1
  • Kenneth Salsman
    • 1
  1. 1.The Saul R. Korey Department of Neurology and the Department of BiochemistryAlbert Einstein College of MedicineBronx

Personalised recommendations