, Volume 5, Issue 9, pp 734–742 | Cite as

The labeling of brain ethanolamine phosphoglycerides from cytidine diphosphate ethanolamine in vitro

  • G. Porcellati
  • M. G. Biasion
  • M. Pirotta


Chicken brain microsomes convert14C-ethanolamine-labeled cytidine diphosphate ethanolamine (CDPE) to ethanolamine lipids (EPG) at a measurable rate. Mitochondria and particle-free supernatant fractions are almost inactive. On adding saturating amounts of diacyl glycerol to the microsomes, formation of EPG increases 10-fold, and is almost totally confined to the diacylsn-glycero-3-phosphorylethanolamine (GPE), whose synthesis increases 20-fold (from 13.1 to 249 mμmoles/mg protein/hr). The addition of the 1-alkenyl 2-acyl glycerol also produces a noticeable increase in the rate of EPG labeling, which is almost exclusively confined to the alkenyl acyl GPE, whose synthesis is stimulated 30-fold (from 3.1 to 90 mμmoles/mg protein/hr). Synthesis of alkyl acyl GPE from CDPE was not detected in the brain microsomes. Synthesis of EPG from CDPE by chicken brain homogenates has also been examined and results similar to those reported for the brain microsomes were obtained. In addition, small amounts of labeled alkyl acyl GPE are produced from CDPE in the homogenates, but in no case does this synthesis increase on adding various lipid acceptors to the incubation system. Various properties of the phosphorylethanolaminediacyl glycerol phosphotransferase (E.C. of brain microsomes were examined. It is concluded that the enzymic activities which convert CDPE to diacyl GPE and alkenyl acyl GPE, respectively, are similar.


Diglyceride Brain Homogenate Glyceryl Ether Soybean Lecithin Chicken Brain 


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  1. 1.
    Ansell, G. B., and S. Spanner, J. Neurochem. 14:873–885 (1967).PubMedCrossRefGoogle Scholar
  2. 2.
    Kennedy, E. P., L. F. Borkenhagen and S. W. Smith, J. Biol. Chem. 234:1998–2000 (1959).PubMedGoogle Scholar
  3. 3.
    McMurray, W. C., J. Neurochem. 11:287–299 (1964).PubMedCrossRefGoogle Scholar
  4. 4.
    Strickland, K. P., D. Subrahmanyam, E. T. Pritchard, W. Thompson and R. J. Rossiter, Biochem. J. 87:128–136 (1963).PubMedGoogle Scholar
  5. 5.
    Ansell, G.B., and R.F. Metcalfe, Ibid. Biochem. J. 98:22P (1966).Google Scholar
  6. 6.
    Bickerstaffe, R., and J. F. Mead, Biochemistry 6:655–662 (1967).PubMedCrossRefGoogle Scholar
  7. 7.
    Horrocks, L. A., and G. B. Ansell, Lipids 2:329–333 (1967).Google Scholar
  8. 8.
    Ansell, G. B., and R. F. Metcalfe, Biochem. J. 109:29P (1968).Google Scholar
  9. 9.
    Porcellati, G., M. G. Biasion, and G. Arienti, Lipids 5:725–733 (1970).PubMedGoogle Scholar
  10. 10.
    Kates, M., T. H. Chan and N. Z. Stanacev, Biochemistry 2:394–397 (1963).CrossRefGoogle Scholar
  11. 11.
    Schneider, W. C., W. G. Fiscus and J. A. B. Lawler, Anal. Biochem. 14:121–134 (1966).CrossRefGoogle Scholar
  12. 12.
    Horrocks, L. A., J. Lipid Res. 9:469–472 (1968).PubMedGoogle Scholar
  13. 13.
    Dawson, R. M. C., N. Hemington and J. B. Davenport, Biochem. J. 84:497–501 (1962).PubMedGoogle Scholar
  14. 14.
    Horrocks, L. A., 4th Meeting of the Federation of European Biochemical Societies, Oslo, 1967, Commun. N. 177.Google Scholar
  15. 15.
    Dawson, R. M. C., Biochem. J. 75:45–53 (1960).PubMedGoogle Scholar
  16. 16.
    Chojnacki, T., and T. Korzybski, Acta Biochim. Polon. 11:341–350 (1964).Google Scholar
  17. 17.
    Schneider, W. C., and R. M. Bekhi, J. Biol. Chem. 238:3565–3571 (1963).PubMedGoogle Scholar
  18. 18.
    Ansell, G. B., and T. Chojnacki, Biochem. J. 98:303–310 (1966).PubMedGoogle Scholar
  19. 19.
    Ansell, G. B., T. Chojnacki and R. F. Metcalfe, J. Neurochem. 12:649–656 (1965).PubMedCrossRefGoogle Scholar
  20. 20.
    Horrocks, L. A., and G. B. Ansell, Biochim. Biophys. Acta 137:90–97 (1967).PubMedGoogle Scholar
  21. 21.
    Kennedy, E. P., and S. B. Weiss, J. Biol. Chem. 222:193–214 (1956).PubMedGoogle Scholar
  22. 22.
    McCaman, R. E., and K. Cook, Ibid. 241:3390–3394 (1966).PubMedGoogle Scholar
  23. 23.
    Schneider, W. C., Ibid. 238:3572–3578 (1963).PubMedGoogle Scholar
  24. 24.
    Snyder, F., in “Progress in the Chemistry of Fats and Other Lipids,” Edited by R. T. Holman, Pergamon Press, London, 1969, p. 289–335.Google Scholar
  25. 25.
    Kuyasu, J. Y., and E. P. Kennedy, J. Biol. Chem. 235:2590–2594 (1960).Google Scholar
  26. 26.
    Snyder, F., R. L. Wylke and B. Malone, Biochem. Biophys. Res. Commun. 34:315–321 (1969).PubMedCrossRefGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1969

Authors and Affiliations

  • G. Porcellati
    • 1
  • M. G. Biasion
    • 1
  • M. Pirotta
    • 1
  1. 1.Department of Biological ChemistryThe University of PaviaItaly

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