, Volume 6, Issue 6, pp 431–433 | Cite as

A possible block in the intermediary metabolism of glucose into proteins and lipids in the brains of undernourished rats

  • H. C. Agrawal
  • M. A. Fishman
  • A. L. Prensky
Short Communications


[U-14C] Glucose or [1-14C] L-leucine was injected intraperitoneally into 28-day-old undernourished rats and control sibs who were killed 6 hr later. Brain proteins and lipids were extracted and the lipids fractionated by silicic acid column chromatography into cholesterol, glycolipids and phospholipids. The specific activity of labeled carbon derived from [U-14C] glucose in brain proteins was reduced by 25% in undernourished animals when compared to controls. A similar reduction was seen in the specific activity of brain lipids of undernourished animals: 14% for cholesterol, 21% for phospholipids and 35% for glycolipids. When [1-14C] l-leucine was used as a direct precursor of brain protein synthesis, the specific activity in the undernourished group was only 5% less than that found for the controls. This was not statistically significant. The results suggest that there may be a block in the intermediary metabolism of glucose in the brains of undernourished rats that reduces the availability of glucose carbon to the precursor pool used for protein and lipid synthesis.


Brain Protein Label Carbon Silicic Acid Column Chromatography Glucose Carbon Globoid Cell Leukodystrophy 
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  1. 1.
    Cremer, J.E., J. Neurochem. 11:165–185 (1964).PubMedCrossRefGoogle Scholar
  2. 2.
    Gaitonde, M.K., and D. Richter, Ibid. 13:1309–1318 (1966).PubMedCrossRefGoogle Scholar
  3. 3.
    Van Den Berg, C.J., Ibid. 17:973–983 (1970).PubMedCrossRefGoogle Scholar
  4. 4.
    Burton, R.M., M.A. Sodd, and R.O. Brady, J. Biol. Chem. 233:1053–1060 (1958).PubMedGoogle Scholar
  5. 5.
    Vrba, R., M.K. Gaitonde, and D. Richter, J. Neurochem. 9:465–475 (1962).PubMedCrossRefGoogle Scholar
  6. 6.
    Smith, M.E., Biochim. Biophys. Acta 164:285–293 (1968).PubMedGoogle Scholar
  7. 7.
    Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall, J. Biol. Chem. 193:265–275 (1951).PubMedGoogle Scholar
  8. 8.
    Folch, J., M. Lees, and G.H. Sloane Stanley, Ibid. 226:497–509 (1957).PubMedGoogle Scholar
  9. 9.
    Rouser, G., G. Kritchevsky, and A. Yamamoto, in “Lipid Chromatographic Analysis”, Edited by G. Marinetti, Marcel Dekker Inc., New York, 1967, p. 116.Google Scholar
  10. 10.
    Zlatkis, A., B. Zak, and A.J. Boyle, J. Lab. Clin. Med. 41:486–492 (1953).PubMedGoogle Scholar
  11. 11.
    Hess, H.H., and E. Lewin, J. Neurochem. 12:205–211 (1965).PubMedCrossRefGoogle Scholar
  12. 12.
    Rouser, G., A.N. Siakotos, and S. Fleischer, Lipids 1:85–86 (1966).Google Scholar
  13. 13.
    Agrawal, H.C., A.H. Bone, and A.N. Davison, Biochem. J. 117:325–331 (1970).PubMedGoogle Scholar
  14. 14.
    Baléazs, R., W. Cocks, J.T. Eayrs and S. Kovacs, in “Hormones in Development”, Edited by M. Hamburgh and E.J.W. Barrington, Appleton-Century-Crofts, New York, in press.Google Scholar
  15. 15.
    Chase, H.P., J. Dorsey and G.M. McKhann, Pediatrics 40:551–560 (1967).PubMedGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1971

Authors and Affiliations

  • H. C. Agrawal
    • 1
  • M. A. Fishman
    • 1
  • A. L. Prensky
    • 1
  1. 1.St. Louis Children’s HospitalMissouriSt. Louis

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