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Effects of renal factors on in vitro hepatic cholesterol synthesis in the rat

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Lipids

Abstract

Two products derived from rat renal tissue have been shown to affect in vitro hepatic cholesterol synthesis. A premevalonate inhibitor of hepatic cholesterol synthesis is associated with the membranes of the renal endoplasmic reticulum. It is stable at −75 C and at 4 C but is heat labile. A pre-mevalonate stimulator of in vitro hepatic cholesterol synthesis is located within renal lysosomes and can be prepared in a nonsedimentable form by extraction with hypotonic buffer. While it is stable at −75 C, it loses activity at 4 C. Both of these products appear to have a molecular weight in excess of 150,000 as determined by gel filtration.

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Authors and Affiliations

  1. Departments of Biochemistry and Pathology, The University of Nebraska College of Medicine, 42nd and Dewey Avenue, Omaha, 68105, Nebraska

    Guy T. Haven (Nebraska Heart Association Post-Doctoral Fellow) & Herbert P. Jacobi

Authors
  1. Guy T. Haven
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  2. Herbert P. Jacobi
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Deceased.

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Haven, G.T., Jacobi, H.P. Effects of renal factors on in vitro hepatic cholesterol synthesis in the rat. Lipids 6, 751–757 (1971). https://doi.org/10.1007/BF02531302

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  • DOI: https://doi.org/10.1007/BF02531302

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