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Lipids

, Volume 6, Issue 7, pp 508–516 | Cite as

Metabolism of ceramide phosphorylethanolamine, phosphatidylinositol, phosphatidylserine and phosphatidylglycerol by housefly larvae

  • G. R. Hildenbrandt
  • T. Abraham
  • L. L. Bieber
Article

Abstract

Microsome preparations (40,000–90,000 g sediment) fromMusca domestica, housefly, larvae convert exogenous32P-labeled phosphatidylinositol, phosphatidylserine and phosphatidylglycerol to the respective lysoglycerophosphatides and, ultimately, to the glycerophosphoryl derivatives. These data, combined with previous results, demonstrate that housefly larvae can convert their normal diacylglycerophosphatides to the respective glycerophosphoryl derivatives. Experiments utilizing exogenous3H-labeled,32P-labeled and14C-labeled ceramide phosphorylethanolamine demonstrate that particulate preparations from housefly larvae convert ceramide phosphorylethanolamine to ceramide, phosphorylethanolamine, sphingosine and fatty acid. The presence of ceramide phosphorylethanolamine phosphohydrolase and ceramidase activity in housefly larvae is consistent with the conclusion that ceramide phosphorylethanolamine is metabolized to ceramide and phosphorylethanolamine and the ceramide is then hydrolyzed to sphingosine and fatty acid. Thus, metabolism of ceramide phosphorylethanolamine by these insects is analogous to the metabolism of sphingomyelin by mammalian systems.

Keywords

Ceramide Sphingosine Water Soluble Fraction Lauryl Sulfate Thin Layer Chromatogram 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© American Oil Chemists’ Society 1971

Authors and Affiliations

  • G. R. Hildenbrandt
    • 1
  • T. Abraham
    • 1
  • L. L. Bieber
    • 1
  1. 1.Department of BiochemistryMichigan State UniversityEast Lansing

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